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Your Position: Home > Gene Products > Other Species > Cynomolgus monkey CD40LG Gene cDNA Clone / ORF Clone

Cynomolgus monkey CD40LG Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
CG90096-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD40LG cDNA Clone Datasheet

CD40LG cDNA Clone Product Information

cDNA Size:	786
Gene Synonym:	CD40LG
Source:	Cynomolgus monkey

CD40LG cDNA Clone Description

cDNA Description:	ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD40 ligand DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 643 G/A resulting in the amino acid Ala substitution by Thr. Please check the sequence information before order.

CD40LG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD40LG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD40LG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCGAAA CATACAACCA ACCTTCTCCC CGATCTGCGG CCACTGGACT GCCCGTCAGA
61       ATGAAAATTT TTATGTATTT ACTTACTATT TTTCTTATCA CCCAGATGAT TGGGTCAGCA
121      CTTTTTGCTG TGTATCTTCA TAGAAGGTTG GACAAGATAG AAGATGAAAG GAATCTTCAT
181      GAAGATTTTG TATTCATGAA AACGATACAG AGATGCAACA CAGGAGAAAG ATCCTTATCC
241      TTACTGAACT GTGAGGAGAT TAAAAGCCAG TTTGAAGGCT TCGTGAAGGA TATAATGCTA
301      AACAAAGAGG AGAAGAAGAA AGAAAACAGC TTTGAAATGC AAAAAGGTGA TCAGAATCCT
361      CAAATTGCGG CACATGTCAT AAGTGAGGCC AGTAGTAAAA CAACATCTGT ACTACAGTGG
421      GCTGAAAAAG GATACTACAC CATGAGCAAC AACTTGGTAA CCCTGGAAAA TGGGAAACAG
481      CTGACCGTTA AAAGACAAGG ACTCTATTAT ATCTATGCCC AAGTCACCTT CTGTTCCAAT
541      CGGGAAGCTT CGAGTCAAGC TCCATTTATA GCCAGCCTCT GCCTAAAGTC CCCCGGTAGA
601      TTCGAGAGAA TCTTACTCAG AGCTGCAAAT ACCCACAGTT CCACCAAACC TTGCGGGCAA
661      CAATCCATTC ACTTGGGAGG AGTATTTGAA TTGCAACCAG GTGCTTCGGT GTTTGTCAAT
721      GTGACTGATC CAAGCCAAGT GAGCCATGGC ACTGGCTTCA CGTCCTTTGG CTTACTCAAA
781      CTCTGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine