|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD40 ligand DNA.|
|Identical with NM_001194367.1 [ Macaca mulatta (Rhesus monkey) ]: 643 G/A resulting in the amino acid Ala substitution by Thr. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey CD40LG Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90096-G-F|
|Cynomolgus monkey CD40LG Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90096-G-H|
|Cynomolgus monkey CD40LG Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90096-G-M|
|Cynomolgus monkey CD40LG Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90096-G-N|
|Cynomolgus monkey CD40LG Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90096-G-Y|
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The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.