Mouse CD40L / TNFSF5 ORF Expression Clone, untagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG50327-M-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD40L / TNFSF5 cDNA Clone Datasheet

CD40L / TNFSF5 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_011616.2
cDNA Size:	783
Gene Synonym:	IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; Tnfsf5; Cd40lg
Source:	Mouse

CD40L / TNFSF5 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus CD40 ligand DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

CD40L / TNFSF5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD40L / TNFSF5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD40L / TNFSF5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATAGAAA CATACAGCCA ACCTTCCCCC AGATCCGTGG CAACTGGACT TCCAGCGAGC
61       ATGAAGATTT TTATGTATTT ACTTACTGTT TTCCTTATCA CCCAAATGAT TGGATCTGTG
121      CTTTTTGCTG TGTATCTTCA TAGAAGATTG GATAAGGTCG AAGAGGAAGT AAACCTTCAT
181      GAAGATTTTG TATTCATAAA AAAGCTAAAG AGATGCAACA AAGGAGAAGG ATCTTTATCC
241      TTGCTGAACT GTGAGGAGAT GAGAAGGCAA TTTGAAGACC TTGTCAAGGA TATAACGTTA
301      AACAAAGAAG AGAAAAAAGA AAACAGCTTT GAAATGCAAA GAGGTGATGA GGATCCTCAA
361      ATTGCAGCAC ACGTTGTAAG CGAAGCCAAC AGTAATGCAG CATCCGTTCT ACAGTGGGCC
421      AAGAAAGGAT ATTATACCAT GAAAAGCAAC TTGGTAATGC TTGAAAATGG GAAACAGCTG
481      ACGGTTAAAA GAGAAGGACT CTATTATGTC TACACTCAAG TCACCTTCTG CTCTAATCGG
541      GAGCCTTCGA GTCAACGCCC ATTCATCGTC GGCCTCTGGC TGAAGCCCAG CAGTGGATCT
601      GAGAGAATCT TACTCAAGGC GGCAAATACC CACAGTTCCT CCCAGCTTTG CGAGCAGCAG
661      TCTGTTCACT TGGGCGGAGT GTTTGAATTA CAAGCTGGTG CTTCTGTGTT TGTCAACGTG
721      ACTGAAGCAA GCCAAGTGAT CCACAGAGTT GGCTTCTCAT CTTTTGGCTT ACTCAAACTC
781      TAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine