Mouse CD40L / CD154 / TNFSF5 Protein (Cytokine)

CD40 ligand Protein Datasheet

CD40L / CD154 / TNFSF5 Protein Product Information

• Synonym: RP23-153G22.3, CD154, CD40-L, Cd40l, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, TRAP, Tnfsf5, gp39
• Protein Construction: A DNA sequence encoding the soluble form of mouse CD40LG (P27548) (Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus.
• Source: Mouse
• Expression Host: Human Cells

CD40L / CD154 / TNFSF5 Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized mouse CD40L-Fc (Cat:50327-M01H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse CD40-Fch (Cat:50324-M03H), The EC₅₀ of biotinylated mouse CD40-Fch (Cat:50324-M03H) is 33-49 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD40L / CD154 / TNFSF5 protein

CD40L / CD154 / TNFSF5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD40L / CD154 / TNFSF5 Protein Description

CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. CD40L ( CD154 / TNFSF5 ) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L ( CD154 / TNFSF5 ) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L ( CD154 / TNFSF5 ) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.

References

1. Korthaeuer U. et al., 1993, Nature. 361: 539-41.
2. Lin Q. et al., 1996, J Clin Invest. 97: 196-201.
3. Seyama K. et al.,1998, Blood. 92: 2421-34.
4. Khandekar SS. et al., 2001, Protein Expr Purif. 23: 301-10.
5. Furman MI. et al., 2004, J Am Coll Cardiol. 43: 2319-25.