CD40L / CD154 / TNFSF5 Antibody (Cytokine)

CD40L / CD154 / TNFSF5 Antibody Datasheet

CD40L / CD154 / TNFSF5 Antibody Product Information

• Immunogen: Recombinant Human CD40L / CD154 / TNFSF5 protein (Catalog#10239-H08E)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 202
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD40L / CD154 / TNFSF5 (rh CD40L / CD154 / TNFSF5; Catalog#10239-H08E; NP_000065.1; Glu 108-Leu 261).

CD40L / CD154 / TNFSF5 Antibody Usage Guide

• Specificity: Human CD40L / CD154 / TNFSF5
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD40L. The detection limit for Human CD40L is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD40L / CD154 / TNFSF5 Antibody Background

CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to thetumor necrosis factor family. CD40L (CD154 / TNFSF5) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L (CD154 / TNFSF5) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L (CD154 / TNFSF5) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.

References

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