|Catalog||Size (Price)||Quantity||In Stock||Operation||Other Information|
CD40L / CD154 / TNFSF5 Antibody Datasheet
|Order or Inquire for CD40L / CD154 / TNFSF5 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.00975 ng/well in ELISA|
CD40L / CD154 / TNFSF5 Antibody Product Information
Recombinant Human CD40L / CD154 / TNFSF5 protein (Catalog#10239-H08E)
|Antibody Type :||Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD40L / CD154 / TNFSF5 (rh CD40L / CD154 / TNFSF5; Catalog#10239-H08E; NP_000065.1; Glu 108-Leu 261).
CD40L / CD154 / TNFSF5 Antibody Usage Guide
Human CD40L / CD154 / TNFSF5
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD40L. The detection limit for Human CD40L is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD40L / CD154 / TNFSF5 Antibody Related Products & Topics
CD40L / CD154 / TNFSF5 Antibody Background
CD40 ligand, also known as CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, CD154, CD40L and TNFSF5, is a single-pass type II membrane protein which belongs to thetumor necrosis factor family. CD40L (CD154 / TNFSF5) is specifically expressed on activated CD4+ T-lymphocytes. The soluble form of CD40L (CD154 / TNFSF5) is derived from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. CD40L (CD154 / TNFSF5) mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. It is involved in immunoglobulin class switching. Defects in CD40L are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1), also known as X-linked hyper IgM syndrome (XHIM). It is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes.
- Korthaeuer U. et al., 1993,Nature 361: 539-41.
- Lin Q. et al., 1996, J. Clin. Invest. 97: 196-201.
- Seyama K. et al.,1998, Blood 92: 2421-34.
- Khandekar S.S. et al., 2001, Protein Expr. Purif. 23: 301-10.
- Furman M.I. et al., 2004, J. Am. Coll. Cardiol. 43: 2319-25.