Rat CD40 Gene cDNA Clone / ORF Clone

CD40 cDNA Clone Datasheet

CD40 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_134360.1
• cDNA Size: 870
• Gene Synonym: Tnfrsf5, Cd40
• Source: Rat

CD40 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD40 molecule, TNF receptor superfamily member 5 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 368 C/G resulting in the amino acid thr substitution by arg and 423 T/C not causing the amino acid variation.

CD40 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD40 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD40 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCCTT TGCCTCAGCT GTGCGCGCTC TGGGGCTGCT TGTTGACAGC GGTCCATCTA
61       GGACAGTGTG TTACGTGCAG TGACAAACAG TACCTCCAAG GTGGCGAGTG CTGCGATTTG
121      TGCCAGCCGG GAAACCGACT AGTTAGCCAC TGCACAGCTC TTGAGAAGAC CCAATGCCAA
181      CCGTGCGACT CAGGCGAATT CTCAGCTCAC TGGAACAGGG AGATCCGCTG CCACCAGCAC
241      CGACACTGCG AACTCAATCA AGGGCTTCAG GTTAAGAAGG AGGGCACCGC GGTTTCAGAC
301      ACTGTTTGTA CCTGCAAGGA AGGGCAGCAC TGCGCCAGCA AGGAGTGCGA GACGTGCGCT
361      CAGCACAGGC CCTGTGGCCC TGGCTTTGGA GTCGTGCAGA TGGCCACTGA GACTACTGAT
421      ACCGTCTGCC AACCCTGCCC GGTCGGATTC TTCTCCAATG GGTCATCACT TTTTGAAAAG
481      TGTCATCCAT GGACAAGCTG TGAAGATCAG AAGCTGATGG TCCTACGGGA AGGAACAAGT
541      CAGACTGATA CCCTCTGTGG TTTCCAGCCC CGGATGCGAG CCCTGCTGGT CATTCCCGTC
601      GTGATGGTCA TCCTCATCAC CATCTTTGTG GTTTTTCTCT ATATCAAAAA GGTGGTCAAG
661      AAACCAAAGG ACAATGAGGT CTTACCCCCT GAGGCTCGAG GACAAGATCC TGTGGAGATA
721      GAAGATTATC CCGGTCACAA CACTGCTGCT CCAGTGCAGG AGACGTTGCA TGGGTGTCAG
781      CCTGTTGCAC AGGAGGATGG TAAAGAGAGT CGCATCTCAG TGCAGGAGCG GCAGGTGACA
841      GGCAGCATGG CCTTGAAGCC CCTGGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.