Human CD40 / TNFRSF5 ORF Expression Clone, FLAG-tagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10774-M-F	pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD40 / TNFRSF5 cDNA Clone Datasheet

CD40 / TNFRSF5 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001250.4
cDNA Size:	861
Gene Synonym:	p50; Bp50; CDW40; MGC9013; TNFRSF5
Source:	Human

CD40 / TNFRSF5 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens CD40 molecule, TNF receptor superfamily member 5 DNA.
Vector:	pCMV/hygro-FLAG
FLAG Tag Sequence:	GATTACAAGGATGACGACGATAAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

CD40 / TNFRSF5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD40 / TNFRSF5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD40 / TNFRSF5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA
61       GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
121      TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
181      CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
241      AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
301      ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
361      CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
421      ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
481      TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
541      AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
601      ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG
661      AAGCCAACCA ATAAGGCCCC CCACCCCAAG CAGGAACCCC AGGAGATCAA TTTTCCCGAC
721      GATCTTCCTG GCTCCAACAC TGCTGCTCCA GTGCAGGAGA CTTTACATGG ATGCCAACCG
781      GTCACCCAGG AGGATGGCAA AGAGAGTCGC ATCTCAGTGC AGGAGAGACA GGCT¹GATTAC
841      AAGGATGACG ACGATAAG²TA A

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2.FLAG Tag

Quality Control:cDNA sequencing results is available upon request.

Cytokine