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CD40 / TNFRSF5 / Bp50 Antibody, Mouse MAb

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Human CD40 Antibody Product Information
Immunogen:Recombinant Human CD40 protein (Catalog#10774-H08H)
Clone ID:2B11B7
Ig Type:Mouse IgG2b
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD40 extracellular domain (rh CD40; Catalog#10774-H08H; NP_001241.1; Met 1-Arg 193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CD40 Antibody Usage Guide
Specificity:Human CD40 / TNFRSF5 / Bp50
No cross-reactivity in ELISA with
Human CD27 / TNFRSF7
Mouse CD27 / TNFRSF7
Human CD30 / TNFRSF8
Human DR6 / TNFRSF21
Human CD137 / 4-1BB
Human TNFRSF1B / CD120b
Human HVEM / TNFRSF14
Mouse HVEM / TNFRSF14
Human RELT / TNFRSF19L
Human cell lysate (293 cell line)
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD40. The detection limit for Human CD40 is 0.0195 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other CD40 Antibody Products
Reactivity: Human  
Application: FCM  
  • Slide 1
10774-MM03-A-50
10774-MM03-A-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
10774-MM03-P-50
10774-MM03-P-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  IF  ICC/IF  
  • Slide 1
  • Slide 1
10774-MM17-200
10774-MM17-100
10774-MM17-50
200 µg 
100 µg 
50 µg 
Add to Cart
Reactivity: Human  
Application: FCM  IF  ICC/IF  
  • Slide 1
  • Slide 1
10774-MM03-100
10774-MM03-50
100 µg 
50 µg 
Add to Cart
Reactivity: Human  
Application: ELISA  
    10774-RP01-400
    10774-RP01-100
    10774-RP01-200
     
    100 µg 
    200 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  WB  
    • Slide 1
    10774-RP02-200
    10774-RP02-50
    10774-RP02-100
    200 µg 
    50 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10774-R004-50
      10774-R004-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10774-MM03-F-50
      10774-MM03-F-100
      50 Tests 
      100 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10774-MM17-A-100
      10774-MM17-A-50
      100 Tests 
      50 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10774-MM17-F-100
      10774-MM17-F-50
      100 Tests 
      50 Tests 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      10774-MM17-P-100
      10774-MM17-P-50
      100 Tests 
      50 Tests 
      Add to Cart
      Reactivity: Human  
      Application: WB  IP  
      • Slide 1
      • Slide 1
      101510-T36-100
      101510-T36-50
      101510-T36-200
      100 µg 
      50 µg 
      200 µg 
      Add to Cart
      Reactivity: Rat  
      Application: ELISA  
        80151-RP01-400
        80151-RP01-200
        80151-RP01-100
         
        200 µg 
        100 µg 
        Add to Cart
        Reactivity: Rat  
        Application: FCM  
        • Slide 1
        80151-MM01-200
        80151-MM01-100
        80151-MM01-50
        200 µg 
        100 µg 
        50 µg 
        Add to Cart
        Reactivity: Rat  
        Application: FCM  
        • Slide 1
        80151-MM01-F-50
        80151-MM01-F-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Rat  
        Application: FCM  
        • Slide 1
        80151-MM01-P-100
        80151-MM01-P-50
        100 Tests 
        50 Tests 
        Add to Cart
        Reactivity: Rat  
        Application: ELISA  
          80151-T16-200
          80151-T16-50
          80151-T16-100
          200 µg 
          50 µg 
          100 µg 
          Add to Cart
          Reactivity: Cynomolgus  
          Application: ELISA  
            90097-T16-200
            90097-T16-50
            90097-T16-100
            200 µg 
            50 µg 
            100 µg 
            Add to Cart
            Reactivity: Rhesus  
            Application: ELISA  
              90097-RP01-400
              90097-RP01-200
              90097-RP01-100
               
              200 µg 
              100 µg 
              Add to Cart
              CD40/TNFRSF5 Background

              CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

              Human CD40/TNFRSF5 References
            • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
            • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
            • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
            • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
            • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
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              All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
              Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"