Ferret CD4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
FG60003-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD4 cDNA Clone Datasheet

CD4 cDNA Clone Product Information

Gene Bank Ref.ID:	EF492055.1
cDNA Size:	1389
Gene Synonym:	CD4
Source:	Ferret

CD4 cDNA Clone Description

cDNA Description:	ORF Clone of Ferret CD4 antigen DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CD4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATCTAG GAGCTGCTCT TAGGCATCTG CTCCTGGTGC TGGAGTTGGC GATGCTCCCC
61       GTGGTCACTC AGGGGAGAGA AGTGGTGTTG GGGAAGGTGG GAGACACAGC CGAGCTGCCG
121      TGCAATGGTT CCGTGGGGCA GAACATAGTC TTCAACTGGA TGCAGTCCAC GGTCAAGATC
181      CTGGGGCGTC AGATCAACTT CTGGATCACA GGTGCCTCCA AGCTGAAGAA TCGTGTGGAA
241      TCGAAAAAAA ACCTGTGGGA CCAAGGATCC TTCCCTCTGA TTATCAAGAA TCTTGAAGCA
301      GCAGACTCGG GGGTTTACTT CTGTGAGGTA GATGGCAAGA AGCAAGCAGT GGAGCTGCTG
361      GTGTTCAACC TGACTGCCAA GTGGAACACC GGCAGCAGCA GCGGCGGCAG CAACATCCGC
421      CTGCTGCAAG GGCAGCAGCT GACCTTGACC GTGGAGGCCC CCTCTGGTAG TAGCCCTTCC
481      GTGCAATGGA AAGGTCCAGG GAATAAAAGC AAGGGCAGTG GCCACAGACT CTCGCTGTCC
541      GGGCTGGACG TGCAGGAGAG CGGGACCTGG ACATGCACCA TCTCCCAGAA CCAGAAGACA
601      GTGGTGTTCA ACATAAACAT CTTGGTGCTG GGTTTCCAGA AGGTCTCTAA CACAGTCTAT
661      TCAAGAGAAG GGGAGCAGGT GAAGTTCTCC TTCCCACTTA ACTTCGAGAT TGAAAAGCTG
721      AGCGGAGAGC TGAGGTGGCG GACAGAGGGC GCTCCCTCCT CCCTGCTCTG GAGCTCCTTC
781      ACCTTGGAGA ACAAGAAGCT ATTTGTCAAA GAGGTCCACC ACCCCAGACT GCAGCTGAAG
841      GAAACGCTCC CACTCAGCTT TATCCTGCCC CAGGCTTCGT CTCAGTATGC TGGTTCTGGA
901      ACCCTGACCC TGAGTCTTGC CAAGGGGACA CTGCAGCAGG AAGTGAATCT GGTGGTGATG
961      CGAGTGACGA AGTTTACGAA CAATTTGACC TGTGAGGTGA TGGGACCCAC CTCCCCGGAG
1021     CTGACGCTGA GCTTGAATCT GGAAGAGCGG GCTGCCAAGG TCTCGAAGCA GCAGAAGCTG
1081     GTGTCGGTGG TGGAGCCCGA GGACGGAACA TGGCAGTGCC TACTGAGTGA CAAAGACAAA
1141     GTCCTGCTGG CCTCCAAGGT TGAGGTTTTG TCCCCAGTGT TCACCAAGGC CTGGCCAAAG
1201     CTCTTGGCCA TTGTGCTGGG CGGGATCTTA GGTCTTCTGC TTCTCCTTGG GCTCTGCATC
1261     TTCTGCTGTA TTAAGTGCTG GCACCGCAGG CGGCAGGCAG CACGGATGTC TCAGATCAAG
1321     AGACTCCTCA GTGAGAAGAA AACCTGCCAA TGCCCCCACC GGCTCCAGAA GGCATGCAAT
1381     CTCATCTGA

Quality Control:cDNA sequencing results is available upon request.