CD4 Protein & Antibody (L3T4)

Cluster of Differentiation 4

CD4 Products

CD4 Protein, Recombinant

Molecule	Species	Description	Cat. No
CD4	Human	CD4/Fc Protein, Recombinant	10400-H03H
CD4	Human	CD4 Protein, Recombinant	10400-H08H
CD4	Human	CD4 (aa 1-208) Protein, Recombinant	10400-H08H1
CD4	Mouse	CD4 Protein, Recombinant	50134-M08H
CD4	Ferret	CD4 Protein, Recombinant	60003-F08H

  10400-H03H:  Measured by its ability to bind with HIV-1 gp120 in a functional ELISA.

  10400-H08H:

1. Measured by its ability to bind with HIV-1 gp120 in a functional ELISA.

2. When 5 x 10⁴ cells/well are added to CD4 coated plates (2.5 µ/mL and 100 µL/well), approximately 60-80% will adhere specifically after 60 minutes at 37℃. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.

  10400-H08H1:

1. Measured by its binding ability in a functional ELISA.

2. When 5 x 10⁴ cells/well  are added to CD4 coated plates (2.5 µg/mL and 100 µL/well), approximately 40 %-60 % will adhere specifically after 60 minutes at 37℃. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.

  50134-M08H: When  5 x 10⁴ cells/well  are added to CD4 coated plates (2.5 µg/mL and 100 µL/well), approximately 40 %-60 % will adhere specifically after 60 minutes at 37 ℃ Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.

  60003-F08H: When 5 x 10⁴ cells/well  are added to CD4 coated plates (2.5 µg/mL and 100 µL/well), approximately 40%-60% will adhere specifically after 60 minutes at 37℃.Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.

CD4 Antibody

Molecule	Application	Description	Cat. No
Human CD4	WB, ELISA	CD4 Antibody, Rabbit Mab	10400-R104
Human CD4	WB FCM	CD4 Antibody ( FITC )	10400-R005-F
Human CD4	ELISA	CD4 / LEU3 Antibody	10400-MM03
Human CD4	WB	CD4 / LEU3 Antibody	10400-MM04
Human CD4	WB, ELISA	CD4 Antibody	10400-RP01
Human CD4	WB, ELISA	CD4 Antibody (Antigen Affinity Purified)	10400-RP02
Mouse CD4	WB, ELISA	CD4 Antibody, Rabbit MAb	50134-R001
Mouse CD4	WB, ELISA	Rabbit Polyclonal Antibody	50134-RP01
Mouse CD4	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50134-RP02
Ferret CD4	WB, ELISA	CD4 Antibody	60003-MM09
Ferret CD4	WB, ELISA	CD4 Antibody (Antigen Affinity Purified)	60003-RP02

CD4 cDNA Clone

Molecule	Species	Description	Cat. No
CD4	Human	CD4 cDNA Clone / ORF Clone	HG10400-M
CD4	Mouse	CD4 cDNA Clone / ORF Clone	MG50134-M
CD4	Ferret	CD4 cDNA Clone / ORF Clone	FG60006-G
CD4	Rat	CD4 cDNA Clone / ORF Clone	RG80086-G

 

CD4 Related Areas

Immunology>>Adaptive Immunity>>T Cell>>Regulatory T Cell / T Cell Antigen Recognition>>CD4

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Lineage Markers>>CD4

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Helper T Cells / Regulatory T Cells / T Cell Antigen Recognition>>CD4

CD4 Alternative Names

CD4 [Homo sapiens]

Cd4, L3T4, Ly-4 [Mus musculus]

CD4 Background

T-cell surface glycoprotein CD4,  is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors.

CD4 Related Studies

1. Farrar WL, et al. (1988) Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development. Crit Rev Immunol. 8(4): 315-39.
2. Biddison WE, et al. (1989) CD4 expression and function in HLA class II-specific T cells. Immunol Rev. 109: 5-15.