CD4 Antibody ( FITC )

CD4 Antibody Datasheet

CD4 Antibody Product Information

• Immunogen: Recombinant Human CD4 protein (Catalog#10400-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 005
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD4 (rHuman CD4; Catalog#10400-H08H; Met 1-Trp 390; NP_000607.1) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD4 Antibody Usage Guide

• Specificity: Human CD4
• No cross-reactivity in ELISA with
  

  Mouse CD4

  Human CD32a

  Human CD32b

  Human CD16a

  Human CD16b

  Human CD64

• Flow Cytometry:
  

  Flow cytometric analysis of anti-human CD4 on human whole blood lymphocytes. Human lymphocytes were stained with FITC-anti human CD4-R005 (solid line) or FITC- rabbit IgG isotype control (dashed line).

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD4 Antibody Background

T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoproteinexpressed on the surface of T helper cells, regulatory T cells,monocytes, macrophages, anddendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 before being named CD4 in 1984. CD4 is aco-receptorthat assists theT cell receptor (TCR) to activate its T cell following an interaction with anantigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly withMHC class II molecules on the surface of the antigen presenting cell via itsextracellulardomain. CD4 is a primary receptor used byHIV-1to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

References

1. Ryu SE, et al., 1994, Structure2 (1): 59-74.
2. Isobe M, et al., 1986, Proc. Natl. Acad. Sci.83 (12): 4399-402.
3. Ansari-Lari MA, et al., 1996, Genome Res.6 (4): 314-26.
4. Hawash, et al., 2002, Biochim. Biophys. Acta. 1589 (2): 140-50.