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Mouse CD3E Gene cDNA Clone (full-length ORF Clone)
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Mouse CD3E Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
MG50410-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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CD3E cDNA Clone Datasheet

 

CD3E cDNA Clone Product Information

Gene Bank Ref.ID: NM_007648.4

cDNA Size:

570

Gene Synonym:

CD3; T3e; AI504783; CD3epsilon; Cd3e

Source: Mouse

CD3E cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus CD3 antigen, epsilon polypeptide DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD3E cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD3E cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD3E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGTGGA ACACTTTCTG GGGCATCCTG TGCCTCAGCC TCCTAGCTGT TGGCACTTGC
61       CAGGACGATG CCGAGAACAT TGAATACAAA GTCTCCATCT CAGGAACCAG TGTAGAGTTG
121      ACGTGCCCTC TAGACAGTGA CGAGAACTTA AAATGGGAAA AAAATGGCCA AGAGCTGCCT
181      CAGAAGCATG ATAAGCACCT GGTGCTCCAG GATTTCTCGG AAGTCGAGGA CAGTGGCTAC
241      TACGTCTGCT ACACACCAGC CTCAAATAAA AACACGTACT TGTACCTGAA AGCTCGAGTG
301      TGTGAGTACT GTGTGGAGGT GGACCTGACA GCAGTAGCCA TAATCATCAT TGTTGACATC
361      TGTATCACTC TGGGCTTGCT GATGGTCATT TATTACTGGA GCAAGAATAG GAAGGCCAAG
421      GCCAAGCCTG TGACCCGAGG AACCGGTGCT GGTAGCAGGC CCAGAGGGCA AAACAAGGAG
481      CGGCCACCAC CTGTTCCCAA CCCAGACTAT GAGCCCATCC GCAAAGGCCA GCGGGACCTG
541      TATTCTGGCC TGAATCAGAG AGCAGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.


 

 

 

CD3e (CD3 epsilon) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"