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CD3e / CD3 epsilon Antibody, Mouse MAb

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    Human CD3E Antibody Product Information
    Immunogen:Recombinant Human CD3E protein (Catalog#10977-H08H)
    Clone ID:1A7E5G5
    Ig Type:Mouse IgG1
    Concentration:
    Formulation:0.2 μm filtered solution in PBS
    Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3E extracellular domain (rh CD3E; Catalog#10977-H08H; Met 1-Asp 126; NP_000724.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
    Other CD3E Antibody Products
    Reactivity: Human  
    Application: FCM  Func/A  
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    10977-H001-500
    10977-H001-100
    10977-H001-1
    500 µg 
    100 µg 
    1 mg 
    Add to Cart
    Reactivity: Human  
    Application: FCM  
    • Slide 1
    • Slide 1
    10977-M001-F-25
    10977-M001-F-100
    25 Test 
    100 Tests 
    Add to Cart
    Reactivity: Human  
    Application: FCM  
    • Slide 1
    10977-M001-C-25
    10977-M001-C-100
    25 Test 
    100 Tests 
    Add to Cart
    Reactivity: Human  
    Application: WB  IP  
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    10977-R801-50
    10977-R801-100
    50 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: FCM  
    • Slide 1
    10977-M001-50
    10977-M001-200
    10977-M001-100
    50 µg 
    200 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10977-R822-50
      10977-R822-100
      50 µg 
      100 µg 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      • Slide 1
      10977-M001-A-25
      10977-M001-A-100
      25 Test 
      100 Tests 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  
        10977-T16-50
        10977-T16-200
        10977-T16-100
        50 µg 
        200 µg 
        100 µg 
        Add to Cart
        Reactivity: Human  
        Application: FCM  
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        10977-M001-P-25
        10977-M001-P-100
        25 Test 
        100 Tests 
        Add to Cart
        Reactivity: Human  
        Application: WB  
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        100216-T32-50
        100216-T32-200
        100216-T32-100
        50 µg 
        200 µg 
        100 µg 
        Add to Cart
        Reactivity: Human  
        Application: ELISA  
          10977-RP01-400
          10977-RP01-200
          10977-RP01-100
          400 µg 
          200 µg 
          100 µg 
          Add to Cart
          Reactivity: Human  
          Application: 
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          GMP-10977-H001-100
          GMP-10977-H001-1
          100 µg 
          1 mg 
          Add to Cart
          Reactivity: Human  
          Application: ELISA  IP  
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          10977-T20-50
          10977-T20-200
          10977-T20-100
          50 µg 
          200 µg 
          100 µg 
          Add to Cart
          Reactivity: Human  
          Application: 
          • Slide 1
          CT026-R301-F-25
          CT026-R301-F-100
          25 Test 
          100 Tests 
          Add to Cart
          Reactivity: Human  
          Application: 
          • Slide 1
          CT026-R301-A-25
          CT026-R301-A-100
          25 Test 
          100 Tests 
          Add to Cart
          CD3e/CD3 epsilon Background

          T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

          Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

          Human CD3e/CD3 epsilon References
        • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
        • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
        • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
        • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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          Size / Price
          Catalog: 10977-MM03-50
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          50 µL
          100 µL
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          Datasheet & Documentation

          All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
          Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"