Anti-CD3D Antibody

Cat: 10981-RP02
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Anti-CD3D Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD3D Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD3D
Recombinant Human CD3D protein (Catalog#10981-H08H)
Produced in rabbits immunized with purified, recombinant Human CD3D (rh CD3D; Catalog#10981-H08H; NP_000723.1; Met 1-Ala 105). CD3D specific IgG was purified by human CD3D affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD3D Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD3D Antibody (Rabbit Polyclonal antibody) Images
Human CD3d/CD3 delta Immunoprecipitation(IP) 8037

CD3D was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg HuT78 Whole Cell Lysate

0.5 µL anti-CD3D rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD3D rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 20 kDa

Observed band size: 20 kDa

Human CD3d/CD3 delta Western blot (WB) 13314

Anti-CD3D rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hu-T78 Whole Cell Lysate

Lysates/proteins at 20 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:19 kDa

Observed band size:21 kDa

Anti-CD3D Antibody Alternative Names
Anti-CD3 delta Antibody;Anti-CD3-DELTA Antibody;Anti-IMD19 Antibody;Anti-T3D Antibody
CD3D Background Information

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD3d molecule, delta (CD3-TCR complex)
  • Roifman CM. (2004) CD3 delta immunodeficiency. Curr Opin Allergy Clin Immunol. 4(6): 479-84.
  • Pan Q, et al. (2006) Different role for mouse and human CD3delta/epsilon heterodimer in preT cell receptor (preTCR) function: human CD3delta/epsilon heterodimer restores the defective preTCR function in CD3gamma- and CD3gammadelta-deficient mice. Mol Immunol. 43(11): 1741-50.
  • Le Deist F, et al. (2007) Expression anomalies of the CD3-TCR complex expression and immunodeficiencies. Med Sci (Paris). 23(2): 161-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD3D Antibody Rabbit Polyclonal WB 102294-T32
    Anti-CD3D Antibody Rabbit Polyclonal ELISA 10981-RP01
    Anti-CD3D Antibody Mouse Monoclonal IHC-P 10981-MM06
    Anti-CD3D Antibody Mouse Monoclonal ELISA(Cap) 10981-MM07
    Anti-CD3D Antibody Mouse Monoclonal WB,IP 10981-MM12
    Anti-CD3D Antibody Mouse Monoclonal ELISA 10981-MM16
    Anti-CD3D Antibody Rabbit Monoclonal WB,IP 10981-R138
    Anti-CD3D Antibody Mouse Monoclonal WB,ELISA,ELISA(Det),IP 10981-MM08
    Anti-CD3D Antibody Rabbit Monoclonal WB,ELISA,IP 10981-R006
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