Rat CD3G Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80314-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD3G cDNA Clone Datasheet

CD3G cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001077646.1
cDNA Size:	549
Gene Synonym:	Cd3g
Source:	Rat

CD3G cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD3 molecule, gamma DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 144 C/G resulting in the amino acid Phe substitution by Leu.

CD3G cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD3G cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD3G cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCAGG GGAAAGGTTT GGCTGGCCTC TTCCTGGTGA TCTCTCTTCT TCAAGGCACC
61       ATGGCCCAGC AAAAAGAAGA AAAGCATTTG GTAAAAGTGG ATGACAGCCA AGGAGATGGC
121      TCTGTACTTC TGACTTGTGA CTTGAATGAG AAGACTATCA CATGGCTTAA AGATGGGCAC
181      AGAATAAGTC CTCCAAATGC AACTAAAAGC ACGTGGAATC TGGGCAACGG TGCCAAAGAC
241      CCTCGAGGCA TGTATCAGTG CCGAGGAGCA AAGAAGAAGT CGCAGCTCCT GCAAGTGTAC
301      TACAGACTGT GTGAGAACTG CATTGAGCTA AATATGGGCA CTGTGTCCGG CTTTATCTTC
361      GCTGAAATCA TCAGCATTTT CTTCCTTGCT GTTGGTGTAT ACTTCATTGC TGGACAGGAT
421      GGAGTTCGCC AGTCAAGAGC TTCAGACAAG CAGACTCTGT TGCAAAATGA ACAGGTCTAC
481      CAGCCCCTCA AGGACCGGGA ATATGAACAG TACAGCCGTC TCCAAGGAAA CCAAGTGAGA
541      AAGAAGTGA

Quality Control:cDNA sequencing results is available upon request.