- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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Molecule, epsilon (CD3-TCR complex) Protein Datasheet
CD3E Protein Price Inquiry ( Available Sizes )
CD3E Protein Product Information
A DNA sequence encoding the human CD3E ( Met 1-Asp126) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
|Expression Host:||Human Cells|
CD3E Protein QC Testing
|Purity:||(84.6+ 14.1) % as determined by SDS-PAGE||SDS-PAGE:
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Gln 22|
The secreted recombinant human CD3E is a disulfide-linked homodimeric protein. The reduced monomer comprises 355 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, it migrates as an approximately 44-53 and 38 kDa band in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile PBS, pH 7.4.
CD3E Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
CD3E Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|CD3e/CD3 epsilon||Human||CD3E Protein, Recombinant||10977-H03H|
|CD3e/CD3 epsilon||Human||CD3e Protein, Recombinant||10977-H08H|
|CD3e/CD3 epsilon||Cynomolgus||CD3E Protein, Recombinant||90047-C02H|
|CD3e/CD3 epsilon||Cynomolgus||CD3E Protein, Recombinant||90047-C08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||CD3e/CD3 epsilon Antibody, Mouse MAb||10977-MM03|
CD3E Protein Description
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
- Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
- Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
- Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
- Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
- Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
- Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.