CD3E Protein

Molecule, epsilon (CD3-TCR complex) Protein Datasheet

CD3E Protein Product Information

• Synonym: CD3E
• Protein Construction: A DNA sequence encoding the human CD3E ( Met 1-Asp126) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CD3E Protein QC Testing

• Purity: (84.6+ 14.1) % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 22
• Molecular Mass: The secreted recombinant human CD3E is a disulfide-linked homodimeric protein. The reduced monomer comprises 355 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, it migrates as an approximately 44-53 and 38 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD3E protein

CD3E Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3E Protein Description

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I  membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. This gene has also been linked to a susceptibility to type I  diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

References

1. Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
2. Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
3. Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
4. Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
5. Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
6. Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.