_×
Human CD3e / CD3 epsilon Protein (His & Fc Tag)
  0 products, total $0.+86-400-890-9989      Login |  Register 中文한국어

Browse By Molecule:

Your Position: Home > Recombinant Protein > Human Cell Expressed > Human CD3e / CD3 epsilon Protein (His & Fc Tag)

Human CD3e / CD3 epsilon Protein (His & Fc Tag) PDF Download

Catalog   Size (Price) Quantity In Stock Operation
10977-H03H  
YES   
 

Protein Production & Purification Service | Recombinant Cytokines & Growth Factors

Molecule, epsilon (CD3-TCR complex) Protein Datasheet

 

CD3E Protein Price Inquiry ( Available Sizes )

CD3E Protein Product Information

Synonym : CD3E
Protein Construction:

A DNA sequence encoding the human CD3E ( Met 1-Asp126) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Source: Human
Expression Host: Human Cells

CD3E Protein QC Testing

Purity: (84.6+ 14.1) % as determined by SDS-PAGE SDS-PAGE:
SDS-PAGE

CD3E protein

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal: Gln 22
Molecular Mass:

The secreted recombinant human CD3E is a disulfide-linked homodimeric protein. The reduced monomer comprises 355 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, it migrates as an approximately 44-53 and 38 kDa band in SDS-PAGE under reducing conditions.

Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

CD3E Protein Usage Guide

Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3E Protein Related Products & Topics

Related Areas:

Cancer>>Cancer Stem Cell (CSC)>>CD3e

Immunology>>Adaptive Immunity>>T Cell>>Regulatory T Cell / T Cell Antigen Recognition>>CD3e

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Regulatory T Cells / T Cell Antigen Recognition>>CD3e

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
CD3e/CD3 epsilon Human CD3E Protein, Recombinant 10977-H03H
CD3e/CD3 epsilon Human CD3e Protein, Recombinant 10977-H08H
CD3e/CD3 epsilon Cynomolgus CD3E Protein, Recombinant 90047-C02H
CD3e/CD3 epsilon Cynomolgus CD3E Protein, Recombinant 90047-C08H

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human
CD3e/CD3 epsilon
WB, ELISA CD3e/CD3 epsilon Antibody, Mouse MAb 10977-MM03

CD3E Protein Description

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I  membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. This gene has also been linked to a susceptibility to type I  diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

References

  1. Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
  2. Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
  3. Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
  4. Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
  5. Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
  6. Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.
 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"