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Cynomolgus monkey CD3D Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
CG90046-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD3D cDNA Clone Datasheet

 

CD3D cDNA Clone Product Information

cDNA Size:

516

Gene Synonym:

CD3D

Source: Cynomolgus monkey

CD3D cDNA Clone Description

cDNA Description:

ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD3d molecule, delta (CD3-TCR complex) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 142 A/G resulting in the amino acid met substitution by val.
Please check the sequence information before order.

CD3D cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD3D cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD3D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACATA GCACGTTTCT GTCTGGACTG GTACTGGCTA CCCTTCTCTC CCAAGTGAGC
61       CCCTTCAAGA TACCTGTAGA GGAACTTGAG GACAGAGTGT TTGTGAAATG CAATACCAGC
121      GTCACATGGG TAGAGGGAAC GGTGGGAACA CTGCTCACAA ATAATACAAG ACTGGACCTG
181      GGAAAACGCA TCCTGGACCC ACGAGGAATA TATAGGTGTA ATGGGACAGA TATATACAAG
241      GACAAAGAAT CTGCTGTGCA AGTTCATTAT CGAATGTGCC AGAACTGTGT GGAGCTGGAT
301      CCAGCCACCC TGGCTGGCAT CATTGTCACT GATGTCATTG CCACTCTGCT CCTTGCTTTG
361      GGAGTCTTCT GCTTTGCTGG ACATGAGACT GGAAGGCTCT CTGGGGCTGC CGACACACAA
421      GCTCTATTGA GGAATGACCA GGTCTATCAG CCCCTCCGAG ATCGAGATGA TGCTCAGTAC
481      AGCCGCCTTG GAGGAAACTG GGCTCGGAAC AAGTGA

 

Quality Control:cDNA sequencing results is available upon request.