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Rat CD3D Gene cDNA Clone (full-length ORF Clone)
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Rat CD3D Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80313-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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CD3D cDNA Clone Datasheet

 

CD3D cDNA Clone Product Information

Gene Bank Ref.ID: NM_013169.1

cDNA Size:

522

Gene Synonym:

Cd3d

Source: Rat

CD3D cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus CD3 molecule, delta DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD3D cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD3D cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD3D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCACT ATGGAATTCT GGTTAGTCTG CTACTGGCTA CTGTTCTCCC CCAAGGGAGC
61       CCCTTCAAGA TAGAAGTGGT TGAATATGAG GACAAAGTGT TTGTGAATTG CAACACCAGC
121      ATCAGGCATC TAGATGGATC AGTGGAAAGA TGGTTGACCA AAAATAAATC ACTCATCTTG
181      GGCAAAGGCA TCCTGGACCC ACGAGGGATG TATATGTGTA ATGGGACAGA GGAGCTGGCG
241      AAGGAGGTGT CGACTGTTCA AGTATATTAC CGAATGTGCC AGAACTGTGT GGAGCTGGAC
301      TCAGCCACCC TGGCTGGTGT CATCATCACT GATCTCATCG CCACTCTGCT CCTGGCTTTG
361      GGGGTCTACT GCTTTGCAGG CCATGAGACC GGAAGACTTT CTGGGGCTGT TGACACTCAA
421      GTCCTGTTGA AGAACGAGCA GCTGTATCAG CCTCTTCGAG ATCGCGATGA TGCCCAGTAC
481      AGCCGTCTTG GAGGGAACTG GCCCCGGAAC AAAAGGTCTT GA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD3d (CD3 delta) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"