CD3D & CD3E Heterodimer Protein

Heterodimer of CD3 antigen, delta polypeptide &CD3 antigen, epsilon polypeptide Protein Datasheet

CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Product Information

• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1- Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1- Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
• Source: Human
• Expression Host: Human Cells

CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 22& Asp 23
• Molecular Mass: The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 （37.5+40）KDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 KDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer protein

CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Description

T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I  membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation.

References

1. Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
2. Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
3. Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
4. Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
5. Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
6. Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.
7. Salmerón A. et al., 1991, J Immunol. 147 (9): 3047-52.