CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Price Inquiry ( Available Sizes )
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Product Information
||A DNA sequence encoding the extracellular domain (Met 1- Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1- Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein QC Testing
||> 90 % as determined by SDS-PAGE.
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer protein
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||Phe 22& Asp 22
||The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 （38+38）KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 KDa in SDS-PAGE under reducing conditions.
||Lyophilized from sterile PBS , pH 7.4
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Related Products & Topics
CD3D&CD3E / CD3 delta&CD3 epsilon Heterodimer Protein Description
T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation.
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