CD3D / CD3-Delta Protein (flag Tag)

CD3d molecule, delta (CD3-TCR complex) Protein Datasheet

CD3D / CD3-Delta Protein Product Information

• Synonym: CD3D
• Protein Construction: A DNA sequence encoding the human CD3D (Met1-Ala105) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus
• Source: Human
• Expression Host: Human Cells

CD3D / CD3-Delta Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 22
• Molecular Mass: The recombinant human CD3D consists of 335 amino acids and predicts a molecular mass of 37.8 KDa. It migrates as an approximately 47-51 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD3D / CD3-Delta protein

CD3D / CD3-Delta Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3D / CD3-Delta Protein Description

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative / B-cell-positive / NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

References

1. Doucey MA. et al., 2003, J Biol Chem. 278 (5): 3257-64.
2. Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
3. Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
4. Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
5. Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
6. Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.