Rat CD38 Protein (His Tag)

CD38 molecule Protein Datasheet

CD38 Protein Product Information

• Synonym: CD38
• Protein Construction: A DNA sequence encoding the rat CD38 (Q64244)(Trp45-Val303) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD38 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 45
• Molecular Mass: The recombinant rat CD38 comprises 270 amino acids and predicts a molecular mass of 31.2 kDa. The apparent molecular mass of the recombinant protein is approximately 42 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD38 protein

CD38 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD38 Protein Description

CD38, also known as ADP-ribosyl cyclase, is a transmembrane glycoprotein comprising two to four N-linked oligosaccharide chains containing sialic acid residues. It is expressed on the surface of immune cells including CD4+, CD8+, B and natural killer cells. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. CD38 functions in cell adhesion, signal transduction and calcium signaling. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic β cells. CD38 has a key role in neuropeptide release, thereby critically regulating maternal and social behaviours, and may be an element in neurodevelopmental disorders. Defects of CD38 is associated with impaired immune responses, metabolic disturbances, and behavioral modifications, and has been connected to human immunodeficiency virus (HIV) infection, leukemias, myelomas, solid tumors, type I I diabetes mellitus and bone metabolism, as well as some genetically determined conditions. In addition, it was demonstrated that CD38 not only acts as a key modulator of the immune response, but also plays an equally important role as an intrinsic pulmonary component. CD38 as the main cellular NADase in mammalian tissues, and the characterization of its role on the control of cellular NAD levels indicate that CD38 may serve as a pharmacological target for multiple conditions.

References

1. Deaglio S. et al., 2001, Leuk Res. 25 (1): 1-12.
2. Jin D. et al., 2007, Nature. 446 (7131): 41-5.
3. Orciani M. et al., 2008, J Cell Biochem. 105 (3): 905-12.
4. Malavasi F. et al., 2008, Physiol Rev. 88 (3): 841-86.
5. Chini EN. et al., 2009, Curr Pharm Des. 15 (1): 57-63.