Rat CD36 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80263-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD36 cDNA Clone Datasheet

CD36 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_031561.2
cDNA Size:	1419
Gene Synonym:	Fat; MGC91634; MGC108510; Cd36
Source:	Rat

CD36 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD36 molecule (thrombospondin receptor) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 839 G/A resulting in the amino acid Gly substitution by Glu and 870 G/A not causing the amino acid variation.

CD36 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD36 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD36 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTGCG ATCGGAACTG TGGGCTCATT ACTGGAGCCG TTATTGGTGC TGTCCTGGCT
61       GTGTTTGGAG GCATTCTCAT GCCGGTTGGA GACCTACTCA TTGAGAAGAC AATCAAAAGG
121      GAAGTTGTCC TTGAAGAAGG AACCATTGCT TTCAAAAACT GGGTGAAAAC GGGCACCACT
181      GTGTACAGAC AGTTTTGGAT CTTTGACGTG CAAAACCCAG AGGAAGTGGC AAAGAATAGC
241      AGCAAGATCA AGGTTAAACA GAGAGGTCCT TACACATACA GAGTTCGTTA TTTAGCCAAG
301      GAAAATATAA CTCAGGACCC CAAGGACAGC ACTGTCTCTT TTGTACAACC CAATGGAGCC
361      ATCTTTGAGC CTTCACTGTC TGTTGGAACA GAGAATGACA ACTTCACAGT TCTCAATCTG
421      GCTGTGGCAG CTGCACCACA TATCTACACA AACTCATTTG TTCAAGGTGT GCTCAACAGC
481      CTTATCAAAA AGTCCAAGTC TTCTATGTTC CAAACACGAA GTTTGAAGGA ACTCTTGTGG
541      GGTTACAAAG ATCCATTCTT GAGTTTGGTT CCATATCCTA TAAGTACCAC AGTTGGTGTG
601      TTTTATCCTT ACAATAACAC TGTAGATGGA GTTTATAAAG TTTTCAATGG AAAGGATAAC
661      ATAAGCAAGG TTGCCATAAT TGATACCTAT AAAGGGAAAA GGAATTTGTC CTATTGGGAA
721      AGTTATTGCG ACATGATTAA TGGCACAGAT GCAGCCTCCT TTCCACCTTT TGTTGAGAAG
781      TCTCGAACAC TGAGGTTCTT TTCCTCTGAC ATTTGCAGGT CCATCTATGC TGTGTTTGAA
841      TCTGAAGTGA ACCTTAAAGG AATCCCCGTA TACAGATTTG TTCTTCCAGC CAACGCCTTT
901      GCCTCCCCAC TCCAGAACCC AGACAACCAC TGTTTCTGCA CTGAAAAAGT AATCTCAAAT
961      AACTGTACGT CGTATGGTGT GCTGGACATT GGCAAGTGCA AAGAAGGAAA GCCTGTGTAC
1021     ATTTCTCTTC CACATTTCCT ACATGCAAGT CCTGATGTCT CAGAACCTAT CGAAGGCTTG
1081     AATCCTAACG AAGATGAGCA TAGGACATAC TTGGATGTGG AACCCATAAC TGGATTCACT
1141     CTACAGTTTG CAAAACGACT GCAGGTCAAC ATACTGGTCA AGCCAGCTAG AAAAATAGAA
1201     GCACTGAAGA ATCTGAAGAG ACCTTACATT GTACCTATAC TGTGGCTAAA TGAGACTGGG
1261     ACCATCGGCG ATGAGAAAGC AGAAATGTTC AGAAACCAAG TGACCGGGAA AATAAAGCTC
1321     CTGGGCCTGG TTGAGATGGT CTTACTTGGT GTTGGAGTAG TGATGTTTGT TGCTTTTATG
1381     ATTTCATACT GTGCTTGCAG ATCTAAGAAT GGAAAATAA

Quality Control:cDNA sequencing results is available upon request.