Rat CD36 / GP3B Protein

CD36 molecule (thrombospondin receptor) Protein Datasheet

CD36 / GP3B Protein Product Information

• Synonym: CD36
• Protein Construction: A DNA sequence encoding the rat CD36 (Gly30-Lys439) was expressed with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD36 / GP3B Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 30
• Molecular Mass: The recombinant rat CD36/Fc is a disulfide-linked homodimer. The reduced monomer comprises 651 amino acids and has a predicted molecular mass of 73.3 kDa. The apparent molecular mass of the protein is approximately 108 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD36 / GP3B protein

CD36 / GP3B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD36 / GP3B Protein Description

Platelet glycoprotein 4, also known as Leukocyte differentiation antigen CD36, GPIIIB, GP3B, FAT, and CD36, is integral membrane protein found on the surface of many cell types in vertebrate animals. It belongs to the CD36 family and is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 has also been implicated in hemostasis, thrombosis, malaria, inflammation, lipid metabolism and atherogenesis. CD36 seems to have numerous potential physiological functions. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of plasmodium falciparum parasitized erythrocytes and binds long chain fatty acids. CD36 may also function as a cell adhesion molecule and in the transport and/or as a regulator of fatty acid transport. Defects in CD36 are the cause of platelet glycoprotein IV deficiency also known as CD36 deficiency. As a receptor for thrombospondin 1, CD36 plays a role in the regulation of angiogenesis, which may be a therapeutic strategy for controlling the dissemination of malignant neoplasms.

References

1. Oquendo P. et al., 1989, Cell. 58 (1): 95-101.
2. Tandon NN. et al., 1989, J Biol Chem. 264 (13): 7576-83.
3. Silverstein RL. et al., 1992, J Biol Chem. 267 (23): 16607-12.
4. Ge Y. et al., 2005, Lab Hematol. 11 (1): 31-7.