Rat CD34 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80262-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD34 cDNA Clone Datasheet

CD34 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001107202.2
cDNA Size:	1161
Gene Synonym:	Cd34
Source:	Rat

CD34 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD34 molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CD34 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD34 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD34 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCGGTCC ACAGGGGCGC GCGCGCGGGT CTCCTGCTAC CATGGGGCTG GGTAGCTCTC
61       TGCCTGATGA GTCTGCTGCA TCTAAATAAC TTGACTTCTG TTGCCTCGGA GAATTCTACC
121      CAAGGAGTAT TTCCACCAGT TCCTACAAAT GAGTCCACTG AGGAAAGTAT CACATCTAGC
181      ATCCCTGGAA GTACCAGCCA CTACTACTTG ATTGGTCAAG ATAGCAATAA GACCACACCA
241      GCCATCTCAG AGACCACGGT CAACTTTACA GCTACCCCTG GGACCCCTTC AGGCTCTGGA
301      ACTCCACACA CTTTTTCACA ACCACAGACT TACCCAACCG TCACACTGCC TACTACTTCA
361      GACAGTACTT CTACTCCAGA AGTGGAACAC TCCACCTGGA ACACCAGCCT GCCGTCTGTC
421      AATGTTTCTG ATTATTCACC TAATAATGTC AGCTCTGAGA TGATATCAAC CACTGAGCCA
481      TGTGCTCACA CATCATCTTC TGTCCCAAGT ATTATTAAGG GAGACATCAA ATGTTCAGGA
541      ATCCGAGGAG TGCCATTTAC TCAGGGCATC TGCCTGGAAC TAAGTGAAGC ATCTAGTTGT
601      GAGGAGTTTA AGAAGGAAAG GGGAGAAGAG CTAATGCAAA TACTGTGTAA AAAGGAGGAG
661      ACTGAGGCCG ACGCTGGTAC TAGTGTCTGC TCCTTGAATC TAGCCCAGTC TGAGGTTAGG
721      CCCGAGTGTT TGCTGATGGT CTTGGCCAAT AGCACAGAAC TTTCCAGCAA ACTCCAGCTT
781      ATGGAAAAGC ACCAGTCTGA CTTGAGAAAG CTGGGGATCC AAAGCTTCAA TAAACAAGAT
841      CTTGGGAGCC ACCAGAGCTA TTCCCGAAAG ACTCTGATTG CATTGGTTAC CTCGGGAGTC
901      CTGCTGGCCA TCTTGGGCAC CACCGGCTAT TTCCTGATGA ACCGCCGCAG TTGGAGTCCC
961      ACAGGAGAAA GGCTGGGTGA AGACCCTTAT TACACGGAGA ATGGTGGAGG CCAGGGCTAT
1021     AGCTCAGGAC CTGGGACCTC CCCTGAGACT CAGGGAAAGG CCAATGTGAC CCGAGGGGCT
1081     CAGGAGAACG GGACCGGCCA GGCCACTTCC AGAAACGGCC ATTCAGCAAG ACAACATGTG
1141     GTGGCTGACA CAGAACTGTG A

Quality Control:cDNA sequencing results is available upon request.