|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Mus musculus CD34 antigen DNA.|
|pMD18-T Simple Vector|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse CD34 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||MG50589-M-F|
|Mouse CD34 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||MG50589-M-H|
|Mouse CD34 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||MG50589-M-M|
|Mouse CD34 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||MG50589-M-N|
|Mouse CD34 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||MG50589-M-Y|
|Product name||Product name|
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.