Mouse CD34 Gene cDNA Clone / ORF Clone

CD34 cDNA Clone Datasheet

CD34 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_133654.3
• cDNA Size: 1149
• Gene Synonym: AU040960, Cd34
• Source: Mouse

CD34 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus CD34 antigen DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD34 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD34 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD34 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGGTCC ACAGGGACAC GCGCGCGGGG CTCCTGCTGC CATGGCGCTG GGTAGCTCTC
61       TGCCTGATGA GTCTGCTGCA TCTAAATAAC TTGACTTCTG CTACCACGGA GACTTCTACA
121      CAAGGAATAT CCCCATCAGT TCCTACCAAT GAGTCTGTTG AGGAAAATAT CACATCTAGC
181      ATCCCTGGAA GTACCAGCCA CTACTTGATC TATCAGGACA GCAGTAAGAC CACACCAGCC
241      ATCTCAGAGA CTATGGTCAA CTTTACAGTT ACCTCTGGGA TCCCTTCAGG CTCTGGAACT
301      CCACACACTT TTTCACAACC ACAGACTTCC CCAACTGGCA TACTGCCTAC TACTTCAGAC
361      AGTATTTCCA CTTCAGAGAT GACCTGGAAG TCCAGCCTGC CATCTATAAA TGTTTCTGAT
421      TATTCGCCTA ATAATAGCAG CTTTGAGATG ACATCACCCA CCGAGCCATA TGCTTACACA
481      TCATCTTCTG CTCCGAGTGC CATTAAGGGA GAAATCAAAT GCTCTGGAAT CCGAGAAGTG
541      AGGTTGGCCC AGGGTATCTG CCTGGAACTA AGTGAAGCAT CTAGTTGTGA GGAGTTTAAG
601      AAGGAAAAGG GAGAAGATCT AATTCAAATA CTGTGTGAAA AGGAGGAGGC TGAGGCTGAT
661      GCTGGTGCTA GTGTCTGCTC CCTGCTTCTA GCCCAGTCTG AGGTTAGGCC TGAGTGTTTG
721      CTGATGGTCT TGGCCAATAG CACAGAACTT CCCAGCAAAC TCCAGCTTAT GGAAAAGCAC
781      CAATCTGACT TGAGAAAGCT GGGGATCCAA AGCTTCAATA AACAAGATAT CGGGAGCCAC
841      CAGAGCTATT CCCGAAAGAC TCTTATTGCA TTGGTCACCT CTGGAGTTCT GCTGGCCATC
901      TTGGGCACCA CTGGTTATTT CCTGATGAAC CGTCGCAGTT GGAGCCCTAC AGGAGAAAGG
961      CTGGGTGAAG ACCCTTATTA CACGGAGAAT GGTGGAGGCC AGGGCTATAG CTCAGGACCT
1021     GGGGCCTCCC CTGAGACTCA GGGAAAGGCC AATGTGACCC GAGGGGCTCA GGAGAACGGG
1081     ACCGGCCAGG CCACTTCCAG AAACGGCCAT TCAGCAAGAC AACATGTGGT GGCTGACACA
1141     GAACTGTGA

 

Quality Control:cDNA sequencing results is available upon request.