CD32b / FCGR2B Protein (His Tag)

Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b Protein

CD32b / FCGR2B Protein Product Information

• CD32b Synonym: CD32, CD32B, FCG2, FCGR2, FCGR2B, IGFR2
• Protein Construction: A DNA sequence encoding the extracellular domain ( Ala 46 - Pro 217 ) of mature human CD32b ( NP_001002274.1 ) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Human Cells

CD32b / FCGR2B Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  Immobilized recombinant human CD32b at 10 μg/mL ( 100μl/well ) can bind human IgG2 with a linear range of 0.16 - 6.4 μg/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 46
• Molecular Mass: The recombinant mature human CD32b consists of 183 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 20.8 kDa. By SDS-PAGE under reducing conditions, it migrates as an approximately 25 - 30 kDa protein due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD32b protein

CD32b / FCGR2B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32b / FCGR2B Protein Description

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three closely related genes encodes human CD32 class by and designated Fcγ RII A, B, and C which share 94 - 99% amino acid identity in their extracellular domains. Fcγ RII B (CD32b) is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. As the only inhibitory member in human, Fcγ RII B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. The coordinated functioning of activating and inhibitory receptors is necessary for successful and proper initiation, amplification, and termination of immune responses, and accordingly, the Fcγ RII B plays an important negative-regulating role through modulating the signals from activation receptors. Recent study has identified CD32b expression in a variety of B-cell malignancies, suggesting that it is a potential immunotherapeutic target for B-cell lymphoproliferative disorders.

References

1. Brooks,D.G. et al., 1989, J. Exp. Med. 170: 1369-1385.
2. Ravetch, J. et al., 2000, Science. 290: 84-89.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.
4. Rankin, C.T. et al., 2006, Blood. 108: 2384-2391.