CD32b / Fc gamma RIIB Antibody

CD32b / Fc gamma RIIB Antibody Datasheet

CD32b / Fc gamma RIIB Antibody Product Information

• Immunogen: Recombinant Cynomolgus CD32b / Fc gamma RIIB protein (Catalog#90014-C08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3G5E12
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus CD32b / Fc gamma RIIB (Catalog#90014-C08H; AAL92097.1; Met 1-Pro 217). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD32b / Fc gamma RIIB Antibody Usage Guide

• Specificity: Cynomolgus CD32b / Fc gamma RIIB
• No cross-reactivity in ELISA with

  Cynomolgus CD32A

  Human CD32A

  Human CD32B

  Human CD16A

  Human CD16B

  Human CD64

  Human cell lysate (293 cell line)
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32B in WB. Using a DAB detection system, the detection limit for Cynomolgus CD32B is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32B. The detection limit for Cynomolgus CD32B is approximately 0.31 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD32b / Fc gamma RIIB Antibody Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three closely related genes encodes human CD32 class by and designated Fcγ RII A, B, and C which share 94 - 99% amino acid identity in their extracellular domains. Fcγ RII B (CD32b) is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. As the only inhibitory member in human, Fcγ RII B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. The coordinated functioning of activating and inhibitory receptors is necessary for successful and proper initiation, amplification, and termination of immune responses, and accordingly, the Fcγ RII B plays an important negative-regulating role through modulating the signals from activation receptors. Recent study has identified CD32b expression in a variety of B-cell malignancies, suggesting that it is a potential immunotherapeutic target for B-cell lymphoproliferative disorders.

References

1. Brooks,D.G. et al., 1989, J. Exp. Med. 170: 1369-1385.
2. Ravetch, J. et al., 2000, Science. 290: 84-89.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.
4. Rankin, C.T. et al., 2006, Blood. 108: 2384-2391.