Cynomolgus monkey CD32B Gene cDNA Clone / ORF Clone

CD32B cDNA Clone Datasheet

CD32B cDNA Clone Product Information

• cDNA Size: 885
• Gene Synonym: CD32B
• Source: Cynomolgus monkey

CD32B cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Fc fragment of IgG, low affinity IIb, receptor (CD32) DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations 144 G/A and 609 A/G not causing the amino acid variation.
  Please check the sequence information before order.

CD32B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD32B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD32B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAATCC TGTCATTCTT ACCTGTCCTT GCTACTGAGA GTGACTGGGC TGACTGCAAG
61       TCCTCCCAGC CTTGGGGCCA CATGCTTCTG TGGACAGCTG TGCTATTCCT GGCTCCTGTT
121      GCTGGGACAC CTGCAGCTCC CCCAAAGGCT GTGCTGAAAC TCGAGCCCCC GTGGATCAAC
181      GTGCTCCGGG AGGACTCTGT GACTCTGACG TGCGGGGGCG CTCACAGCCC TGACAGCGAC
241      TCCACTCAGT GGTTCCACAA TGGGAATCTC ATCCCCACCC ACACGCAGCC CAGCTACAGG
301      TTCAAGGCCA ACAACAATGA TAGCGGGGAG TACAGGTGCC AGACTGGCCG GACCAGCCTC
361      AGCGACCCTG TTCATCTGAC TGTGCTTTCT GAGTGGCTGG CGCTCCAGAC CCCTCACCTG
421      GAGTTCCGGG AGGGAGAAAC CATCTTGCTG AGGTGCCACA GCTGGAAGGA CAAGCCTCTG
481      ATCAAGGTCA CATTCTTCCA GAATGGAATA TCCAAGAAAT TTTCCCATAT GAATCCCAAC
541      TTCTCCATCC CACAAGCAAA CCACAGTCAC AGTGGTGATT ACCACTGCAC AGGAAACATA
601      GGCTACACGC CATACTCATC CAAACCTGTG ACCATCACTG TCCAAGTGCC CAGCATGGGC
661      AGCTCTTCAC CGATAGGGAT CATTGTGGCT GTGGTCACTG GGATTGCTGT AGCGGCCATT
721      GTTGCTGCTG TAGTGGCCTT GATCTACTGC AGGAAAAAGC GGATTTCAGC CAATCCCACT
781      AATCCTGACG AGGCTGACAA AGTTGGGGCT GAGAACACAA TCACCTATTC ACTTCTCATG
841      CATCCGGACG CTCTGGAAGA GCCTGATGAC CAAAACCGNG TTTAG

 

Quality Control:cDNA sequencing results is available upon request.