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Human CD32b / FCGR2B ELISA Pair Set

  • Human CD32b / FCGR2B ELISA Pair Set
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Human CD32b Materials provided
Capture Ab:0.5 mg/mL of mouse anti-CD32b monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10259-MM01)
Detection Ab:0.5 mg/mL rabbit anti-CD32b monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10259-R112)
Standard:Each vial contains 55 ng of recombinant CD32b. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
Human CD32b Sensitivity
The minimum detectable dose of Human CD32b / FCGR2B was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human CD32b Principle of the product
The human CD32b / FCGR2B ELISA Pair Set is for the quantitative determinationof human CD32b / FCGR2B.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32b / FCGR2B coated on a 96-well plate. Standards and samples are added to the wells, and any CD32b / FCGR2B present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD32b / FCGR2B monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD32b / FCGR2B present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human CD32b Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human CD32b ELISA Pair Set Standard Curve
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Catalog: SEKA10259-15
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