CD32b / FCGR2B Antibody ( FITC )

CD32b / FCGR2B Antibody Datasheet

CD32b / FCGR2B Antibody Product Information

• Immunogen: Recombinant Human CD32b protein (Catalog#10259-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 112
• Ig Type: Rabbit IgG
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD32b / FCGR2B (rh CD32b / FCGR2B; Catalog#10259-H08H; NP_001002274.1; Ala 46-Pro 217) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD32b / FCGR2B Antibody Usage Guide

• Specificity: Human CD32b / FCGR2B
• Has cross-reactivity in ELISA with
  

  Human CD64

  No cross-reactivity in ELISA with
  

  Mouse FCGR2B

  Human CD32a

  Human CD16b

  Human CD16a

• Flow Cytometry: Profile of CD32 reactivity on THP-1 cells analyzed by flow cytometry.
  Cells should be Fc-blocked by treatment with 20 μg of human IgG/10⁶ cells for 1 hour at 4 ℃ prior to staining, washed, then stained with FITC rabbit anti-CD32b (10259-R112).
  
  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD32b in WB. Using a DAB detection system, the detection limit for Human CD32b is approximately 0.5 ng/lane under non-reducing conditions and 0.125 ng/lane under reducing conditions.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD32b / FCGR2B Antibody Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three closely related genes encodes human CD32 class by and designated Fcγ RII A, B, and C which share 94 - 99% amino acid identity in their extracellular domains. Fcγ RII B (CD32b) is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. As the only inhibitory member in human, Fcγ RII B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. The coordinated functioning of activating and inhibitory receptors is necessary for successful and proper initiation, amplification, and termination of immune responses, and accordingly, the Fcγ RII B plays an important negative-regulating role through modulating the signals from activation receptors. Recent study has identified CD32b expression in a variety of B-cell malignancies, suggesting that it is a potential immunotherapeutic target for B-cell lymphoproliferative disorders.

References

1. Brooks,D.G. et al., 1989, J. Exp. Med. 170: 1369-1385.
2. Ravetch, J. et al., 2000, Science. 290: 84-89.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.
4. Rankin, C.T. et al., 2006, Blood. 108: 2384-2391.