0 products, total $0.+86-400-890-9989      Login |  Register 中文한국어

Browse By Molecule:

Your Position: Home > ELISA > ELISA Pair Set > CD32b / FCGR2B ELISA Pair Set , Human ELISA Pair Set

CD32b / FCGR2B ELISA Pair Set , Human ELISA Pair Set PDF Download

Catalog Size (Price) Quantity In Stock Operation Other Information
SEK10259

  YES          

Human CD32b ( Fc-gamma-RIIb / FCGR2B ) ELISA Pair Set

Order or Inquire for Human CD32b / FCGR2B ELISA Pair Set
High affinity Human CD32b / FCGR2B ELISA Pair Set

Cross-React with CD32a / FcRIIA / FCGR2A ( 73% )

Detection limit - 31.25 pg/ml
Affordable price and 30%-80% cost saving for Bulk order

 

  • Materials
  • Protocol
  • Trouble Shooting
  • Image & Flash
     

Problems

Possible Sources

Solutions

No signal

Incorrect or no Detection Antibody was added

Add appropriate Detection Antibody and continue

Substrate solution was not added

Add substrate solution and continue

Incorrect storage condition

Check if the kit is stored at recommended condition and used before expiration date

     

Poor Standard Curve

Standard was incompletely reconstituted or was inappropriately stored

Aliquot reconstituted standard and store at  -70 ℃

Imprecise / inaccurate pipetting

Check / calibrate pipettes

Incubations done at inappropriate temperature, timing or agitation

Follow the general ELISA protocol

Background wells were contaminated

Avoid cross contamination by using the sealer appropriately

     

Poor detection value

The concentration of antigen in samples was too low

Enriching samples to increase the concentration of antigen

Samples were ineffective

Check if the samples are stored at cold environment. Detect samples in timely manner

     

High Background

Insufficient washes

Use multichannel pipettes without touching the reagents on the plate

Increase cycles of washes and soaking time between washes

TMB Substrate Solution was contaminated

TMB Substrate Solution should be clear and colorless prior to addition to wells

Materials were contaminated

Use clean plates, tubes and pipettes tips

     

Non-specificity

Samples were contaminated

Avoid cross contamination of samples

The concentration of samples was too high

Try higher dilution rate of samples

 

 

 

CD32b / FCGR2B ELISA Pair Set Related Products & Topics

Related Areas:

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD32/FCGR2

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Pathogen Recognition/Uptake>>CD32/FCGR2

Immunology>>Cluster of Differentiation>>Granulocyte CD Antigen>>Granulocyte Markers>>CD32/FCGR2

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers>>CD32/FCGR2

Immunology>>Fc Receptor>>CD32/FCGR2

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
CD32/FCGR2 Mouse CD32/FCGR2 Protein, Recombinant 50030-M08H
CD32/FCGR2 Mouse CD32/FCGR2 Protein, Recombinant, with AVI Tag 50030-M27H
CD32/FCGR2 Mouse CD32/FCGR2 Protein, Recombinant, with AVI Tag, Biotinylated 50030-M27H-B
CD32a/FCGR2A Human CD32a/FCGR2A/Fc Protein, Recombinant 10374-H02H
CD32a/FCGR2A Human CD32a/FCGR2A Protein, Recombinant 10374-H08H
CD32a/FCGR2A Human CD32a/FCGR2A Protein, Recombinant 10374-H08B
CD32a/FCGR2A Human CD32a/FCGR2A Protein (167 Arg/His), Recombinant 10374-H08H1
CD32a/FCGR2A Human CD32a/FCGR2A Protein, Recombinant, with AVI Tag 10374-H27H
CD32a/FCGR2A Human CD32a/FCGR2A Protein (167 Arg/His), Recombinant, with AVI Tag 10374-H27H1
CD32a/FCGR2A Human CD32a/FCGR2A Protein, Recombinant, with AVI Tag, Biotinylated 10374-H27H-B
CD32a/FCGR2A Human CD32a/FCGR2A Protein (131 Arg/His), Recombinant, with AVI Tag, Biotinylated 10374-H27H1-B
CD32a/FCGR2A Cynomolgus CD32a/FCGR2A Protein, Recombinant 90015-C08H
CD32a/FCGR2A Cynomolgus CD32a/FCGR2A Protein, Recombinant, with AVI Tag 90015-C27H
CD32a/FCGR2A Cynomolgus CD32a/FCGR2A Protein, Recombinant, with AVI Tag, Biotinylated 90015-C27H-B
CD32a/FCGR2A Cynomolgus CD32a/FCGR2A Protein, Recombinant 90016-C08H
CD32b/FCGR2B Human CD32b/FCGR2B Protein, Recombinant 10259-H08H
CD32b/FCGR2B Human CD32b/FCGR2B Protein, Recombinant with AVI Tag 10259-H27H
CD32b/FCGR2B Human CD32b/FCGR2B Protein, Recombinant, with AVI Tag, Biotinylated 10259-H27H-B
CD32b/FCGR2B Rat CD32b/FCGR2B Protein, Recombinant 80018-R08H
CD32b/FCGR2B Cynomolgus CD32b/FCGR2B Protein, Recombinant 90014-C08H
CD32b/FCGR2B Cynomolgus CD32b/FCGR2B Protein, Recombinant, with AVI Tag 90014-C27H
CD32b/FCGR2B Cynomolgus CD32b/FCGR2B Protein, Recombinant, with AVI Tag, Biotinylated 90014-C27H-B

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Mouse
CD32/FCGR2
WB, ELISA CD32/FCGR2 Antibody, Rabbit MAb 50030-R005
Mouse
CD32/FCGR2
ELISA Rabbit Polyclonal Antibody 50030-RP01
Mouse
CD32/FCGR2
WB, ELISA Rabbit Polyclonal Antibody (Antigen Affinity Purified) 50030-RP02
Human
CD32a/FCGR2A
ELISA Mouse Monoclonal Antibody 10374-MM01
Human
CD32a/FCGR2A
ELISA Mouse Monoclonal Antibody 10374-MM02
Human
CD32b/FCGR2B
WB, ELISA Mouse Monoclonal Antibody
10259-MM02
Human
CD32b/FCGR2B
WB, ELISA CD32b/FCGR2B Antibody, Rabbit Mab
10259-R112
Human
CD32b/FCGR2B
IHC CD32b/FCGR2B Antibody, Rabbit Mab
10259-R110
Human
CD32b/FCGR2B
WB, ELISA Rabbit Polyclonal Antibody 10259-RP01
Human
CD32b/FCGR2B
WB, ELISA Rabbit Polyclonal Antibody (Antigen Affinity Purified) 10259-RP03

Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three closely related genes encodes human CD32 class by and designated Fcγ RII A, Fcγ RII B, and Fcγ RII C which share 94 - 99% amino acid identity in their extracellular domains. Fcγ RII B (CD32b) is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. As the only inhibitory member in human, Fcγ RII B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. The coordinated functioning of activating and inhibitory receptors is necessary for successful and proper initiation, amplification, and termination of immune responses, and accordingly, the Fcγ RII B plays an important negative-regulating role through modulating the signals from activation receptors. Recent study has identified CD32b expression in a variety of B-cell malignancies, suggesting that it is a potential immuno-therapeutic target for B-cell lymphoproliferative disorders.