CD32b / FCGR2B Antibody

Fc-gamma-RIIb ( CD32b / FCGR2B ) Antibody

CD32b / FCGR2B Antibody Product Information

• Immunogen: Recombinant human CD32b protein ( Catalog #10259-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 110
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD32b extracellular domain ( rh CD32b ; Catalog#10259-H08H ; Ala 46 - Pro 217 ; NP_001002274.1 ).

CD32b / FCGR2B Antibody Usage Guide

• Specificity: Human CD32b / FCGR2B
• Has cross-reactivity in ELISA with
  Human CD64
  Human CD16b
  No cross-reactivity in ELISA with
  Human CD32a
  Human CD16a
  Mouse FCGR2B
• Immunochemistry: IHC-P: 0.5-5 μg/mL

  

  Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of B cells and some other mononuclear cell (0.5 μg/mL).

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD32b in WB.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD32b in ELISA.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD32b / FCGR2B Antibody Background

Low affinity immunoglobulin gamma Fc region receptor II-b, also known as IgG Fc receptor II-b, Fc-gamma RII-b, Fc-gamma-RIIb, CD32b and FCGR2B, is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD32b / FCGR2B is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. CD32b / FCGR2B is involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to CD32b / FCGR2B results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor.

An Fc receptor is a protein found on the surface of certain cells ( natural killer cells, macrophages, neutrophils, and mast cells ) that contribute to the protective functions of the immune system. Fc receptors bind to antibodies that are attached to infected cells or invading pathogens. Their activity stimulates phagocytic or cytotoxic cells to destroy microbes, or infected cells by antibody-mediated phagocytosis or antibody-dependent cell-mediated cytotoxicity. There are several different types of Fc receptors, which are classified based on the type of antibody that they recognize. Those that bind the most common class of antibody, IgG, are called Fc-gamma receptors (FcγR), those that bind IgA are called Fc-alpha receptors (FcαR) and those that bind IgE are called Fc-epsilon receptors (FcεR). All Fc-gamma receptors belong to the immunoglobulin superfamily and are the most important Fc receptors. This family includes several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which differ in their antibody affinities due to their different molecular structure.

References

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