Anti-CD32B Antibody

Cat: 10259-R110
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All CD32B Reagents
Anti-CD32B Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD32B Antibody
Validated applications
WB,IHC-P,FCM,IP
Species reactivity
Reacts with: Human
Specificity
Human CD32B
Has cross-reactivity in ELISA with Human CD64 Human CD16b
No cross-reactivity in ELISA with Human CD32a Human CD16a Mouse FCGR2B
Immunogen
Recombinant Human CD32b protein (Catalog#10259-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD32b extracellular domain (rh CD32b; Catalog#10259-H08H; Ala 46-Pro 217; NP_001002274.1).
Source
Monoclonal Rabbit IgG Clone #110
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD32B Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
IHC-P 1:2500-1:15000  
FCM 1:25-1:100  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD32B Antibody (Rabbit Monoclonal antibody) Images
Human CD32b/FCGR2B/Fc gamma RIIB Immunohistochemistry(IHC) 2724
Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of B cells and some other mononuclear cell (1:10000).
Human CD32b/FCGR2B/Fc gamma RIIB Western blot (WB) 13155

Anti-CD32b rabbit monoclonal antibody at 1:500 dilution

Lane A: Daudi Whole Cell Lysate

Lane B: THP1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:38 kDa

Human CD32b/FCGR2B/Fc gamma RIIB Immunoprecipitation(IP) 14042

CD32B was immunoprecipitated using:

Lane A:0.5 mg Daudi Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

1 µL anti-CD32B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD32B rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Human CD32B Flow Cytometry (FC) 19147
Human peripheral blood lymphocytes were stained with Human CD32b Monoclonal Ab (10259-R110), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.Flow cytometric analysis of CD32b expression on human lymphocytes.
Anti-CD32B Antibody Alternative Names
Anti-CD32 Antibody;Anti-CD32B Antibody;Anti-Fc gamma RIIB Antibody;Anti-FCGR2 Antibody
CD32B Background Information
Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Product Description Host Clonality Application Catalog# (PDF)
Anti-CD32B Antibody Mouse Monoclonal ELISA 90014-MM03
Anti-CD32B Antibody (APC) Mouse Monoclonal FCM 90014-MM12-A
Anti-CD32B Antibody Rabbit Monoclonal ELISA,FCM 90014-R046
Anti-CD32B Antibody Mouse Monoclonal IHC-P 90014-MM06
Anti-CD32B Antibody Mouse Monoclonal FCM 90014-MM12
Anti-CD32B Antibody (FITC) Mouse Monoclonal FCM 90014-MM12-F
Anti-CD32B Antibody (FITC) Rabbit Monoclonal FCM 90014-R046-F
Anti-CD32B Antibody (PE) Rabbit Monoclonal FCM 90014-R046-P
Anti-CD32B Antibody (APC) Rabbit Monoclonal FCM 90014-R046-A
Anti-CD32B Antibody Rabbit Polyclonal ELISA 90014-RP01
Anti-CD32B Antibody Rabbit Polyclonal ELISA,IHC-P 90014-RP02
Anti-CD32B Antibody (PE) Mouse Monoclonal FCM 90014-MM12-P
Anti-CD32B Antibody Rabbit Polyclonal WB,ELISA 80018-RP01
Anti-CD32B Antibody Rabbit Polyclonal ELISA,IHC-P 80018-RP02
Anti-CD32B Antibody Rabbit Polyclonal ELISA 10259-RP03
Anti-CD32B Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10259-R112
Anti-CD32B Antibody Mouse Monoclonal ELISA 10259-MM02
Anti-CD32B Antibody Mouse Monoclonal ELISA(Cap) 10259-MM01
info info
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