Cynomolgus CD32a / FCGR2A Protein (His Tag)

Cynomolgus CD32a / FCGR2A Protein

Cynomolgus CD32a / FCGR2A Protein Product Information

• Synonym: FCGR2A
• Protein Construction: A DNA sequence encoding the Cynomolgus ( Macaca fascicularis ) FCGR2A extracellular domain ( Met 1 - Ile 217 ) was fused with a polyhistidine tag at the C-terminus
• Source: Cynomolgus
• Expression Host: Human Cells

Cynomolgus CD32a / FCGR2A Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 34
• Molecular Mass: The recombinant cynomolgus FCGR2A consists of 195 amino acids and has a calculated molecular mass of 21.7 kDa. The apparent molecular mass of the recombinant protein is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD32a protein

Cynomolgus CD32a / FCGR2A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cynomolgus CD32a / FCGR2A Protein Description

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Cynomolgus low affinity immunoglobulin gamma Fc region receptor II-a, also known as IgG Fc receptor II-a, Fc-gamma-RIIa, CD32a and FCGR2A, is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD32a / FCGR2A is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM (immunoreceptor tyrosine-based activation motif)- bearing adapter subunit, FcRγ, CD32a / FCGR2A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as FcγRIIB, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

References

1. Stuart, S.G. et al., 1989, EMBO. J. 8: 3657-66.
2. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-83.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-92.
4. Nagarajan, S. et al., 2000, Blood. 3: 1069-77.