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Rhesus CD32a / Fc gamma RIIA / FCGR2A ELISA Pair Set

  • Rhesus CD32a/FCGR2A/Fc gamma RIIA ELISA Pair Set Standard Curve 15124
  • Cynomolgus CD32a / Fc gamma RIIA / FCGR2A ELISA standard curve
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Rhesus CD32a Materials provided
Capture Ab:0.5 mg/mL of mouse anti-Rhesus FCGR2A monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 90016-MM09)
Detection Ab:0.25 mg/mL mouse anti-Rhesus FCGR2A monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 90016-MM02)
Standard:Each vial contains 18 ng of recombinant Rhesus FCGR2A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
Rhesus CD32a Sensitivity
The minimum detectable dose of Rhesus CD32a / Fc gamma RIIA / FCGR2A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Rhesus CD32a Principle of the product
The Rhesus CD32a / Fc gamma RIIA / FCGR2A ELISA Pair Set is for the quantitative determination of Rhesus CD32a / Fc gamma RIIA / FCGR2A.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32a / Fc gamma RIIA / FCGR2A coated on a 96-well plate. Standards and samples are added to the wells, and any CD32a / Fc gamma RIIA / FCGR2A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD32a / Fc gamma RIIA / FCGR2A monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD32a / Fc gamma RIIA / FCGR2A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Rhesus CD32a Storage
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Catalog: SEK90016-5
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