|0.5 mg/mL of mouse anti-Rhesus FCGR2A monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 90016-MM09)|
|0.25 mg/mL mouse anti-Rhesus FCGR2A monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 90016-MM02)|
|Each vial contains 18 ng of recombinant Rhesus FCGR2A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.|
|The minimum detectable dose of Rhesus CD32a / Fc gamma RIIA / FCGR2A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|The Rhesus CD32a / Fc gamma RIIA / FCGR2A ELISA Pair Set is for the quantitative determination of Rhesus CD32a / Fc gamma RIIA / FCGR2A.|
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32a / Fc gamma RIIA / FCGR2A coated on a 96-well plate. Standards and samples are added to the wells, and any CD32a / Fc gamma RIIA / FCGR2A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD32a / Fc gamma RIIA / FCGR2A monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD32a / Fc gamma RIIA / FCGR2A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.