CD32a / Fc gamma RIIA / FCGR2A Antibody

CD32a / Fc gamma RIIA / FCGR2A Antibody Datasheet

CD32a / Fc gamma RIIA / FCGR2A Antibody Product Information

• Immunogen: Recombinant Cynomolgus CD32a / Fc gamma RIIA / FCGR2A protein (Catalog#90016-C08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 01
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus CD32a / Fc gamma RIIA / FCGR2A (rC CD32a / Fc gamma RIIA / FCGR2A; Catalog#90016-C08H; Met 1-Ile 217). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD32a / Fc gamma RIIA / FCGR2A Antibody Usage Guide

• Specificity: Cynomolgus CD32a / Fc gamma RIIA / FCGR2A
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Cynomolgus FCGR2A in WB. Using a DAB detection system, the detection limit for Cynomolgus FCGR2A is approximately 4 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Cynomolgus FCGR2A. The detection limit for Cynomolgus FCGR2A is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD32a / Fc gamma RIIA / FCGR2A Antibody Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Low affinity immunoglobulin gamma Fc region receptor II-a, also known as IgG Fc receptor II-a, Fc-gamma-RIIa, CD32a and FCGR2A, is a single-pass type I membrane protein which contains twoIg-like C2-type (immunoglobulin-like) domains. CD32a / FCGR2A is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM (immunoreceptor tyrosine-based activation motif)- bearing adapter subunit, FcRγ, CD32a / FCGR2A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as FcγRIIB, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

References

1. Stuart, S.G. et al., 1989, EMBO. J. 8: 3657-3666.
2. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-283.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.
4. Nagarajan, S. et al., 2000, Blood. 3: 1069-1077.