CD32a / Fc gamma RIIA Antibody (Antigen Affinity Purified)

CD32a / Fc gamma RIIA Antibody ( Antigen Affinity Purified ) Datasheet

CD32a / Fc gamma RIIA Antibody Product Information

• Immunogen: Recombinant Cynomolgus CD32a / Fc gamma RIIA protein (Catalog#90015-C08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Cynomolgus CD32a / Fc gamma RIIA (rC CD32a / Fc gamma RIIA; Catalog#90015-C08H; AAL92096.1; Met 1-Ile 211). CD32a / Fc gamma RIIA specific IgG was purified by Cynomolgus CD32a / Fc gamma RIIA affinity chromatography.

CD32a / Fc gamma RIIA Antibody Usage Guide

• Specificity: Cynomolgus CD32a / Fc gamma RIIA
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32A in WB. Using a DAB detection system, the detection limit for Cynomolgus CD32A is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32A. The detection limit for Cynomolgus CD32A is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD32a / Fc gamma RIIA Antibody Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94 - 99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fcγ RII A is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM (immunoreceptor tyrosine-based activation motif)- bearing adapter subunit, Fc Rγ, CD32a (Fcγ RII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

References

1. Stuart, S.G. et al., 1989, EMBO. J. 8: 3657-3666.
2. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-283.
3. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.
4. Nagarajan, S. et al., 2000, Blood. 3: 1069-1077.