Anti-CD32 Antibody

Cat: 10374-MM01
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Anti-CD32 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD32 Antibody
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human CD32
Has cross-reactivity with Human CD32b
No cross-reactivity with Human CD16a, Human CD16b and Human cell lysate (293 cell line) in ELISA
Immunogen
Recombinant Human CD32a protein (Catalog#10374-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD32a extracellular domain (rh CD32a; Catalog#10374-H08H; Met 1-Ile 218; AAA35827.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #4B7C6A2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD32 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD32 Antibody (Mouse Monoclonal antibody) Images
Human CD32/Fc gamma RII/FCGR2 Flow Cytometry (FC) 17114
Flow cytometric analysis of Human CD32 expression on human whole blood lymphocytes (left panel), monocytes (middle panel) and granulocytes (right panel). Cells were stained with purified anti-Human CD32, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes, monocytes and granulocytes.
Anti-CD32 Antibody Alternative Names
Anti-CD32 Antibody;Anti-CD32A Antibody;Anti-CD32B Antibody;Anti-CDw32 Antibody;Anti-Fc gamma RII Antibody;Anti-FCG2 Antibody;Anti-FcGR Antibody;Anti-FCGR21 Antibody;Anti-FCGR2A Antibody;Anti-IGFR2 Antibody
CD32 Background Information

Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

References
  • Williams TE, et al. (2000) Concurrent and independent binding of Fcgamma receptors IIa and IIIb to surface-bound IgG. Biophys J. 79(4): 1867-75.
  • Kanters D, et al. (2007) Expression of activated Fc gamma RII discriminates between multiple granulocyte-priming phenotypes in peripheral blood of allergic asthmatic subjects. J Allergy Clin Immunol. 120(5): 1073-81.
  • Veri MC, et al. (2007) Monoclonal antibodies capable of discriminating the human inhibitory Fcgamma-receptor IIB (CD32B) from the activating Fcgamma-receptor IIA (CD32A): biochemical, biological and functional characterization. Immunology. 121(3): 392-404.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD32 Antibody Rabbit Monoclonal ELISA 50030-R005
    Anti-CD32 Antibody Rabbit Polyclonal ELISA 50030-RP02
    Anti-CD32 Antibody Rabbit Polyclonal ELISA 50030-T16
    Anti-CD32 Antibody Rabbit Polyclonal ELISA 50030-RP01
    Anti-CD32 Antibody (PE) Mouse Monoclonal FCM 10374-MM01-P
    Anti-CD32 Antibody (FITC) Mouse Monoclonal FCM 10374-MM01-F
    Anti-CD32 Antibody (APC) Mouse Monoclonal FCM 10374-MM01-A
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