CD32a / FCGR2A Antibody

Low Affinity Immunoglobulin gamma Fc Region Receptor II-a Antibody

CD32a / FCGR2A Antibody Product Information

• Immunogen: Recombinant human CD32a protein ( Catalog#10374-H08H )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2C3B11B8
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD32a extracellular domain ( rh CD32a ; Catalog#10374-H08H ; Met 1 - Ile 218 ; AAA35827.1 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD32a / FCGR2A Antibody Usage Guide

• Specificity: Human CD32a
• No cross-reactivity with human CD32b, human CD16a, human CD16b and human cell lysate ( 293 cell line ) in ELISA.
• Direct ELISA: This antibody can be used at 0.5 - 1.0 μg/mL with the appropriate secondary reagents to detect human CD32a . The detection limit for human CD32a is approximately 5 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD32a / FCGR2A Antibody Background

Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Human CD32 class is divided into three closely related proteins, and designated FcγRIIA (CD32a), FcγRIIB (CD32b), and FcγRIIC (CD32c). They share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32a is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. CD32a delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production.

References

1. Stuart, S.G. et al., 1989, EMBO. J. 8: 3657-3666.
2. Nagarajan, S. et al., 2000, Blood. 3: 1069-1077.
3. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-283.
4. Takai, T., 2002, Nature Rev. Immunol. 2: 580-592.