Mouse CD32 / FCGR2B Protein (AVI Tag) , Biotinylated

Mouse FCGR2B / CD32 ( Biotinylated ) Protein

Mouse FCGR2B / CD32 ( Biotinylated ) Protein Product Information

• Synonym: AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, Fcgr2b
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Arg 217 ) of mouse FGGR2B ( NP_001070657.1 ) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase ( BirA enzyme ) which is co-expressed
• Source: Mouse
• Expression Host: Human Cells

Mouse FCGR2B / CD32 ( Biotinylated ) Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• mMB / mMP: 0.80
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Thr 40
• Molecular Mass: The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FCGR2B protein

Mouse FCGR2B / CD32 ( Biotinylated ) Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Mouse FCGR2B / CD32 ( Biotinylated ) Protein Description

FcγRII (CD32) is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. There are three main classes of human leucocyte IgG Fc receptor (FcγR): FcγRI (CD64), FcγRII (CD32), and FcγRШ (CD16) which are distinguished on the basis of molecular weights, cellular distribution, affinity/specificity for ligand and by reactivity with monoclonal antibodies. Many important functions have been attributed to these receptors and they are regarded as providing an essential link between cellular and humoral immune systems. The most ubiquitous of these receptors is FcγRII. The extracellular region of FcγRII comprises two C2-type I g-like disulfide-bonded domains which is structurally conserved in all of the Ig superfamily leukocyte FcRs (and represents an Ig-interactive motif. FcγRII is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

References

1. Hulett, M. D. et al., 1995, J Biol Chem.  270 (36): 21188-21194.
2. Reterink, T. et al., 1996,  J Eur J Immunol. 26 (8):1970-1973.
3. Sandilands, G. P. et al., 1997,  Immunology.   90 (3): 427-434.