Mouse CD32 / FCGR2B Protein (His Tag)

Mouse Fc Receptor, IgG, Low Affinity Iib Protein Datasheet

FCGR2B / CD32 Protein Price Inquiry ( Available Sizes )

500μg:  Inquiring Price;   ≥1mg Bulk:  Inquiring Price

FCGR2B / CD32 Protein Product Information

• FCGR2B Synonym: AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1, Fcgr2b
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Arg 217 ) of mouse FCGR2B ( NP_001070657.1 ) precursor was expressed with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

FCGR2B / CD32 Protein QC Testing

• Purity: > 98% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃.
• Predicted N terminal: Thr 40
• Molecular Mass: The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 35 - 40 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD32 protein

FCGR2B / CD32 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FCGR2B / CD32 Protein Description

FcRII (CD32) is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. There are three main classes of human leucocyte IgG Fc receptor (FcR) : FcRI (CD64), FcRII (CD32), and FcR (CD16) which are distinguished on the basis of molecular weights, cellular distribution, affinity/specificity for ligand and by reactivity with monoclonal antibodies. Many important functions have been attributed to these receptors and they are regarded as providing an essential link between cellular and humoral immune systems. The most ubiquitous of these receptors is FcRII. The extracellular region of FcRII comprises two C2-type I g-like disulfide-bonded domains which is structurally conserved in all of the Ig superfamily leukocyte FcRs (and represents an Ig-interactive motif. FcRII is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

References

1. Hulett, M. D. et al., 1995, J Biol Chem. 270 (36): 21188-21194.
2. Reterink, T. et al., 1996, J Eur J Immunol. 26 (8):1970-1973.
3. Sandilands, G. P. et al., 1997, Immunology. 90 (3): 427-434.