FCGR2B / CD32 Antibody

Mouse FCGR2B / CD32 Antibody

FCGR2B / CD32 Antibody Product Information

• Immunogen: Recombinant mouse CD32 protein ( Catalog#50030-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 005
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse FCGR2B / CD32 ( rM FCGR2B ; Catalog#50030-M08H ; Met 1 - Ala 217 ; NP_001070657.1 ).

FCGR2B / CD32 Antibody Usage Guide

• Specificity: Mouse FCGR2B / CD32
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse FCGR2B in WB. Using a DAB detection system, the detection limit for mouse FCGR2B is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse FCGR2B. The detection limit for mouse FCGR2B is approximately 0.0049 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FCGR2B / CD32 Antibody Background

FcγRII (CD32) is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. There are three main classes of human leucocyte IgG Fc receptor (FcγR): FcγRI (CD64), FcγRII (CD32), and FcγRⅢ (CD16) which are distinguished on the basis of molecular weights, cellular distribution, affinity/specificity for ligand and by reactivity with monoclonal antibodies. Many important functions have been attributed to these receptors and they are regarded as providing an essential link between cellular and humoral immune systems. The most ubiquitous of these receptors is FcγRII. The extracellular region of FcγRII comprises two C2-type Ig-like disulfide-bonded domains which is structurally conserved in all of the Ig superfamily leukocyte FcRs (and represents an Ig-interactive motif. FcγRII is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

References

1. Hulett, MD. et al., 1995, J Biol Chem.  270 (36): 21188-94.
2. Reterink, T. et al., 1996,  J Eur J Immunol. 26 (8):1970-3.
3. Sandilands, GP. et al., 1997,  Immunology.   90 (3): 427-34.