Anti-CD32 Antibody

Cat: 50030-RP02
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All CD32 Reagents
Anti-CD32 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD32 Antibody
Validated applications
Species reactivity
Reacts with: Mouse
Mouse CD32
Recombinant Mouse CD32 protein (Catalog#50030-M08H)
Produced in rabbits immunized with purified, recombinant Mouse FCGR2B / CD32 extracellular domain (rM FCGR2B; Catalog#50030-M08H; Met 1-Ala 217; NP_001070657.1). FCGR2B specific IgG was purified by mouse FCGR2B affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD32 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

CD32 Background Information

Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

  • Williams TE, et al. (2000) Concurrent and independent binding of Fcgamma receptors IIa and IIIb to surface-bound IgG. Biophys J. 79(4): 1867-75.
  • Kanters D, et al. (2007) Expression of activated Fc gamma RII discriminates between multiple granulocyte-priming phenotypes in peripheral blood of allergic asthmatic subjects. J Allergy Clin Immunol. 120(5): 1073-81.
  • Veri MC, et al. (2007) Monoclonal antibodies capable of discriminating the human inhibitory Fcgamma-receptor IIB (CD32B) from the activating Fcgamma-receptor IIA (CD32A): biochemical, biological and functional characterization. Immunology. 121(3): 392-404.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD32 Antibody Rabbit Monoclonal ELISA 50030-R005
    Anti-CD32 Antibody Rabbit Polyclonal ELISA 50030-T16
    Anti-CD32 Antibody Rabbit Polyclonal ELISA 50030-RP01
    Anti-CD32 Antibody Mouse Monoclonal ELISA,FCM 10374-MM01
    Anti-CD32 Antibody (PE) Mouse Monoclonal FCM 10374-MM01-P
    Anti-CD32 Antibody (FITC) Mouse Monoclonal FCM 10374-MM01-F
    Anti-CD32 Antibody (APC) Mouse Monoclonal FCM 10374-MM01-A
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