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CD31 / PECAM1 Antibody, Mouse MAb

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Human CD31/PECAM1 Antibody Product Information
Immunogen:Recombinant Human CD31 protein (Catalog#10148-H08H)
Clone ID:13
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; Catalog#10148-H08H; Met 1-Lys 601; NP_000433.3). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CD31/PECAM1 Antibody Usage Guide
Specificity:Human CD31 / PECAM1
No cross-reactivity in ELISA with
Human CD54
Human CD62
Human CD106
Human cell lysate (293 cell line)
Application:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD31/PECAM1 Antibody IHC Application Image
CD31 / PECAM-1 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human CD31 in HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD31 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to cell membrane.
Human CD31/PECAM1 Antibody FC Application Image
CD31 / PECAM-1 Antibody, Mouse MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of anti-human CD31 on human whole blood monocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes.
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    Application: ELISA  WB  IHC-P  IP  
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    Reactivity: Human  
    Application: FCM  
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    Reactivity: Human  
    Application: ELISA  IHC-P  
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    10148-R078-50
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    Application: FCM  
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    Application: FCM  
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    Reactivity: Mouse  
    Application: ELISA  IHC-P  
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    Application: ELISA  
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      CD31/PECAM-1 Background

      The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

      Human CD31/PECAM-1 References
    • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
    • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
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      Catalog: 10148-MM13-200
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"