CD31 / PECAM1 Antibody

CD31 / PECAM1 Antibody Datasheet

CD31 / PECAM1 Antibody Product Information

• Immunogen: Recombinant Human CD31 protein (Catalog#10148-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD31 / PECAM1 (rh CD31 / PECAM1; Catalog#10148-H08H; EAW94208.1; Met 1-Lys 601). Total IgG was purified by Protein A affinity chromatography.

CD31 / PECAM1 Antibody Usage Guide

• Specificity: Human CD31 / PECAM1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD31 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD31. The detection limit for Human CD31 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD31 / PECAM1 Antibody Background

The CD31 adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets, and is the only known member of the CAM family on platelets. PECAM-1 has six Ig-homology domains that share sequence similarity with cellular adhesion molecules within the extracellular region, and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Accordingly, CD31acts as a signaling molecule when appropriately engaged and creates docking sites for the Src homology 2 domain-bearing protein tyrosine phosphatase (SHP)-1 and SHP-2. PECAM-1 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

References

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