CD31 / PECAM1 Antibody ( FITC )

CD31 / PECAM1 Antibody Datasheet

CD31 / PECAM1 Antibody Product Information

• Immunogen: Recombinant Human CD31 / PECAM1 protein (Catalog#10148-H08H)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 13
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 (rh CD31 / PECAM1; Catalog#10148-H08H; EAW94208.1; Met 1-Lys 601) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD31 / PECAM1 Antibody Usage Guide

• Specificity: Human CD31 / PECAM1
• Flow Cytometry:
  

  Profile of anti-CD31 reactivity on HUVEC cells analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD31 / PECAM1 Antibody Background

(PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets, and is the only known member of the CAM family on platelets. PECAM-1 has six Ig-homology domains that share sequence similarity with cellular adhesion molecules within the extracellular region, and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Accordingly, CD31acts as a signaling molecule when appropriately engaged and creates docking sites for the Src homology 2 domain-bearing protein tyrosine phosphatase (SHP)-1 and SHP-2. PECAM-1 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

References

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2. Sun, J. et al., 1996, J. Biol. Chem. 271: 18561-18570.
3. Newton-Nash, D.K. and Neman, P.J. et al., 1999, J. Immunol. 163: 682-688.
4. Rui, Y.X. et al., 2007, J. Immunol. 179: 7344-7351.
5. Woodfin, A. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27: 2514-2523.