|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Mus musculus platelet/endothelial cell adhesion molecule 1 DNA.|
|Cd31, Pecam, C85791, PECAM-1, MGC102160, Pecam1|
|pMD18-T Simple Vector|
|Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1464 G/A and 2178 A/C not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||MG50408-M-F|
|Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||MG50408-M-H|
|Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||MG50408-M-M|
|Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||MG50408-M-N|
|Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||MG50408-M-Y|
|Product name||Product name|
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.