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Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone)
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Mouse CD31 / PECAM1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
MG50408-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

PECAM1 cDNA Clone Datasheet

 

PECAM1 cDNA Clone Product Information

Gene Bank Ref.ID: NM_008816.2

cDNA Size:

2184

Gene Synonym:

Cd31; Pecam; C85791; PECAM-1; MGC102160; Pecam1

Source: Mouse

PECAM1 cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus platelet/endothelial cell adhesion molecule 1 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1464 G/A and 2178 A/C not causing the amino acid variation.

PECAM1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PECAM1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PECAM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTCCTGG CTCTGGGACT CACGCTGGTG CTCTATGCAA GCCTCCAGGC TGAGGAAAAC
61 TCCTTCACCA TCAACAGCAT CCATATGGAA AGCCTGCCAT CATGGGAGGT GATGAATGGG
121 CAGCAACTGA CCCTGGAGTG CCTTGTGGAC ATCAGCACCA CCTCGAAAAG CAGGTCTCAG
181 CACCGGGTGC TGTTCTATAA GGACGATGCG ATGGTGTATA ACGTCACCTC CAGGGAGCAC
241 ACCGAGAGCT ACGTCATTCC TCAGGCTCGG GTCTTCCACT CCGGGAAGTA CAAATGCACA
301 GTGATGCTGA ACAACAAGGA AAAAACCACG ATTGAGTACG AGGTGAAGGT GCATGGCGTA
361 TCCAAGCCCA AGGTGACACT GGACAAAAAG GAGGTGACAG AAGGCGGGGT CGTGACGGTC
421 AATTGTTCCT TGCAAGAAGA AAAGCCACCG ATCTTTTTTA AAATTGAAAA ATTAGAAGTG
481 GGGACAAAGT TTGTCAAGCG AAGGATAGAT AAGACCTCCA ACGAGAACTT TGTGCTCATG
541 GAATTCCCCA TTGAGGCGCA GGACCACGTG TTAGTGTTTC GCTGCCAAGC TGGGATCCTG
601 TCCGGATTCA AATTGCAGGA GTCAGAACCC ATCAGGAGTG AATACGTCAC CGTGCAGGAG
661 TCCTTCTCCA CTCCCAAGTT TGAAATCAAG CCCCCTGGGA TGATCATAGA AGGGGACCAG
721 CTGCACATTA GGTGCATAGT TCAAGTGACA CACTTGGTCC AGGAGTTTAC AGAAATTATC
781 ATCCAAAAAG ACAAGGCGAT TGTAGCCACC TCCAAGCAAA GCAGTGAAGC TGTCTACTCA
841 GTCATGGCCA TGGTCGAGTA CAGTGGACAC TACACCTGCA AAGTGGAATC AAACCGTATC
901 TCCAAAGCCA GTAGCATCAT GGTCAACATA ACAGAGCTGT TTCCCAAGCC GAAGTTAGAG
961 TTCTCCTCCA GTCGTCTGGA CCAAGGGGAG TTGTTGGACC TGTCCTGCTC CGTCTCGGGC
1021 ACACCTGTAG CCAACTTCAC CATCCAGAAG GAAGAGACGG TCTTGTCGCA GTATCAGAAT
1081 TTCAGCAAGA TCGCCGAGGA GAGTGACAGC GGGGAGTACA GCTGTACTGC AGGCATCGGC
1141 AAAGTGGTCA AGAGAAGCGG CCTGGTACCG ATCCAGGTGT GCGAAATGCT CTCGAAGCCC
1201 AGTATTTTTC ATGATGCCAA GTCTGAGATC ATAAAAGGAC ATGCCATAGG CATCAGCTGC
1261 CAGTCCGAAA ATGGAACTGC ACCCATCACT TACCACCTTA TGAAAGCAAA GAGTGACTTC
1321 CAGACTCTCG AGGTGACCTC CAATGACCCA GCAACATTCA CAGATAAGCC CACCAGAGAC
1381 ATGGAATACC AGTGCAGAGC GGATAATTGC CATTCCCACC CCGCGGTGTT CAGCGAGATC
1441 CTGAGGGTCA GGGTGATAGC CCCAGTGGAT GAAGTTGTGA TTTCCATCCT GTCGAGTAAC
1501 GAGGTACAGT CTGGAAGTGA AATGGTACTT CGGTGCTCTG TGAAAGAGGG GACGAGCCCA
1561 ATCACGTTTC AGTTTTACAA AGAAAAGGAG GACAGACCCT TCCACCAAGC GGTCGTGAAT
1621 GACACCCAAG CGTTTTGGCA CAACAAACAA GCTAGCAAGA AGCAGGAAGG ACAGTACTAC
1681 TGTACAGCCT CCAACAGAGC CAGCAGTATG AGGACCAGTC CCCGAAGCAG CACTCTTGCA
1741 GTCAGAGTCT TCCTTGCCCC ATGGAAGAAA GGGCTCATTG CGGTGGTTGT CATTGGAGTG
1801 GTCATCGCCA CCTTAATAGT TGCAGCCAAA TGCTACTTCC TGAGGAAAGC CAAGGCCAAA
1861 CAGAAACCCG TGGAGATGTC CAGGCCAGCT GCTCCACTTC TGAACTCCAA CAGCGAGAAG
1921 ATTTCTGAGC CTAGTGTGGA AGCCAACAGC CATTACGGTT ATGATGATGT TTCTGGAAAT
1981 GATGCAGTAA AACCCATAAA TCAAAATAAA GACCCCCAGA ACATGGATGT AGAATACACA
2041 GAAGTGGAAG TGTCCTCCCT TGAGCCTCAC CAAGCTCTGG GAACGAGAGC CACAGAGACG
2101 GTGTACAGTG AGATCCGGAA GGTCGACCCT AATCTCATGG AAAACAGATA CTCTAGAACG
2161 GAAGGCTCCC TTAATGGCAC TTAA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD31/PECAM-1 related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"