Anti-CD31 Antibody

Cat: 10148-MM02
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All CD31 Reagents
Anti-CD31 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD31 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD31
Immunogen
Recombinant Human CD31 protein (Catalog#10148-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; Catalog#10148-H08H; Met 1-Lys 601; NP_000433.3). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #02
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD31 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 0.5-1 μg/mL  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD31 Antibody Alternative Names
Anti-CD31 Antibody;Anti-CD31/EndoCAM Antibody;Anti-endoCAM Antibody;Anti-GPIIA' Antibody;Anti-PECA1 Antibody;Anti-PECAM-1 Antibody
CD31 Background Information

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Full Name
platelet/endothelial cell adhesion molecule 1
References
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-CD31 Antibody Rabbit Monoclonal ELISA 50408-R009
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    Anti-CD31 Antibody Mouse Monoclonal IHC-P,FCM 10148-MM14
    Anti-CD31 Antibody Rabbit Monoclonal IHC-P 10148-R311
    Anti-CD31 Antibody Rabbit Monoclonal ICC/IF,IF 10148-R007
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    Anti-CD31 Antibody (APC) Mouse Monoclonal FCM 10148-MM14-A
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    Anti-CD31 Antibody Mouse Monoclonal ELISA(Det) 10148-MM12
    Anti-CD31 Antibody Mouse Monoclonal FCM,ICC/IF,IF 10148-MM13
    Anti-CD31 Antibody Rabbit Monoclonal IHC-P 10148-R324
    Anti-CD31 Antibody Mouse Monoclonal ELISA(Cap) 10148-MM06
    Anti-CD31 Antibody (PE) Mouse Monoclonal FCM 10148-MM02-P
    Anti-CD31 Antibody Rabbit Monoclonal ELISA,IHC-P 10148-R078
    Anti-CD31 Antibody Mouse Monoclonal ELISA 10148-MM18
    Anti-CD31 Antibody (FITC) Mouse Monoclonal FCM 10148-MM14-F
    Anti-CD31 Antibody (PE) Mouse Monoclonal FCM 10148-MM13-P
    Anti-CD31 Antibody (FITC) Mouse Monoclonal FCM 10148-MM13-F
    Anti-CD31 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,ICC/IF,IF,IP 10148-T62
    Anti-CD31 Antibody Mouse Monoclonal IHC-P 10148-MM28
    Anti-CD31 Antibody (FITC) Mouse Monoclonal FCM 10148-MM02-F
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