Rat CD302 Gene cDNA Clone / ORF Clone

CD302 cDNA Clone Datasheet

CD302 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001013916.1
• cDNA Size: 687
• Gene Synonym: MGC108799; RGD1307606; Cd302
• Source: Rat

CD302 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD302 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 652 T/G resulting in the amino acid Ser substitution by Ala.

CD302 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD302 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD302 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCCACG CAGCGCTATC CTCGCTCGTG CTGCTGAGCC TAGCCACTGC CATCTTCGCT
61       GACTGTCCTT CGTCCATCTG GGTTCAGTTC CAAGGCAGCT GTTACACTTT TCTTCAAGTA
121      ACCATCAATG TGGAAAACAT AGAGGATGTC AGAAAGCAGT GTACTGATCA CGGGGCAGAC
181      CTGGTAAGTA TACACAATGA AGAAGAAAAC GCATTTATAC TGGACACTTT ACAAAAGCGA
241      TGGAAAGGCC CGGATGATCT TCTGCTAGGC ATGTTTTATG ACACTGATGA TGCAAGTTTC
301      AAGTGGTTTG ATCAGTCAAA TATGACATTC GACAAGTGGG CAGATGAGGA TGGTGAGGAC
361      CTAGTTGACA CCTGTGGTTT TCTGTATGCC AAGACAGGTG AATGGAGAAA AGGAAATTGT
421      GAAATGTCTT CTGTGACAGG AACACTTTGC AAAACAGCAA TCCCATATGA CAAGAAGTAT
481      TTATCAGATA ACCACATTTT AATATCGACT CTGGTGATCG CTAGCACAGT GACTCTGGCA
541      GTTTTGGGAG CGGTCATTTG GTTCCTCTAT AGAAGGAGCG CACGCTCTGG CTTCACCTCT
601      TTCTCTCCTG CACCACAATC ACCTTACAGT GATGGCTGTG CTCTGGTAGT TGCGGAAGAA
661      GATGAATACT CTGTTCAGCT GGACTGA

 

Quality Control:cDNA sequencing results is available upon request.