|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus CD302 molecule DNA.|
|MGC108799, RGD1307606, Cd302|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 652 T/G resulting in the amino acid Ser substitution by Ala.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CD302 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80414-G-F|
|Rat CD302 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80414-G-H|
|Rat CD302 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80414-G-M|
|Rat CD302 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80414-G-N|
|Rat CD302 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80414-G-Y|
|Product name||Product name|
CD302/CLEC13A (C-type lectin domain family 13 member A), also known as C-type lectin receptor DCL-1, is a type I transmembrane C-type lectin DCL-1/CD302. DCL-1 protein was highly conserved among the human, mouse, and rat orthologs. DCL-1 ectodomain contains only one CRD, whereas other type I transmembrane C-type lectins contain more than one domain (e.g. selectins and MMR). DCL-1 CP contains several putative motifs, including a Tyr-based internalization, a cluster of acidic amino acids, and Ser and Tyr phosphorylation motifs, suggesting that DCL-1 CP mediates not only endocytosis and late endosome targeting but also signaling. DCL-1 may be another cell/matrix adhesion receptor integrated in cell adhesion complexes and that DCL-1 dysfunction may affect APC adhesion and migration, causing suppression of APC function.