Rat CD302 / CLEC13A Protein

CD302 molecule Protein Datasheet

CD302 / CLEC13A Protein Product Information

• Synonym: CD302
• Protein Construction: A DNA sequence encoding the rat CD302 (Met1-His165) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD302 / CLEC13A Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 21
• Molecular Mass: The recombinant rat CD302/Fc is a disulfide-linked homodimer. The reduced monomer comprises 386 amino acids and has a predicted molecular mass of 43.7 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD302 / CLEC13A protein

CD302 / CLEC13A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD302 / CLEC13A Protein Description

CD302, also known as C-type lectin domain family 13 member A (CLEC13A), or Type I transmembrane C-type lectin receptor DCL-1, is an unconventional C-type lectin that may play roles not only in endocytosis/phagocytosis but also in APC adhesion and migration. DCL-1 protein was highly conserved among the human, mouse, and rat orthologs. DCL-1 CP contains several putative motifs, including a Tyr-based internalization, a cluster of acidic amino acids, and Ser and Tyr phosphorylation motifs, suggesting that DCL-1 CP mediates not only endocytosis and late endosome targeting but also signaling.

References

1. Skinnider B. et al., 2002, Blood. 99(12): 4283-97.
2. Kato M. et al., 2007, J Immunol. 179(9): 6052-63.